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Term merge: GO:0106054 tRNA-uracil-34 sulfurtransferase activity & GO:0103016 tRNA-specific 2-thiouridylase activity #27928

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sjm41 opened this issue May 21, 2024 · 2 comments
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@sjm41
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sjm41 commented May 21, 2024

These look like they may be the same thing? - almost identical defs, synonym of GO:0106054 is the name of GO:0103016.

Only PomBase has EXP annotations to GO:0106054 (ncs6 and ctu2) @ValWood
Only dictyBase has EXP annotation to GO:0103016 (to ctu1). @pfey03

id: GO:0106054
name: tRNA-uracil-34 sulfurtransferase activity
namespace: molecular_function
def: "Catalysis of the reaction: uridine34 in tRNA + a [TusE sulfur carrier protein]-S-sulfanylcysteine + ATP + a reduced electron acceptor = a 2-thiouridine34 in tRNA + a [TusE sulfur carrier protein]-L-cysteine + AMP + an oxidized electron acceptor + diphosphate + H+." [PMID:12549933, PMID:16387657]
synonym: "tRNA U34 2-thiouridylase" EXACT []
synonym: "tRNA U34 thiol-transferase activity" EXACT []
synonym: "tRNA-specific 2-thiouridylase" RELATED []
is_a: GO:0016783 ! sulfurtransferase activity
is_a: GO:0140101 ! catalytic activity, acting on a tRNA
relationship: part_of GO:0002098 ! tRNA wobble uridine modification
property_value: term_tracker_item "#13257" xsd:anyURI
created_by: hjd
creation_date: 2017-09-05T19:10:12Z

id: GO:0103016
name: tRNA-specific 2-thiouridylase activity
namespace: molecular_function
def: "Catalysis of the reaction: ATP + H+ + a tRNA uridine34 + a [TusE sulfur carrier protein]-S-sulfanylcysteine = AMP + diphosphoric acid + a tRNA 2-thiouridine34 + a [TusE sulfur carrier protein]-L-cysteine." [GOC:pz, PMID:12949933]
xref: MetaCyc:RXN0-2023
is_a: GO:0016874 ! ligase activity

@sjm41
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sjm41 commented May 21, 2024

The MetaCyc xref on GO:0103016 (MetaCyc:RXN0-2023) links to EC:2.8.1.13 (= 'tRNA-uridine 2-sulfurtransferase'), which is RHEA:47032):
AH2 + ATP + S-sulfanyl-L-cysteinyl-[protein] + uridine(34) in tRNA = 2-thiouridine(34) in tRNA + A + AMP + diphosphate + H(+) + L-cysteinyl-[protein]

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sjm41 commented May 31, 2024

Thanks @raymond91125
I see the final term has ended up with parentage to both 'transferase activity' and 'ligase activity'. It should just have the 'sulfurtransferase activity' parent (following EC classification) - I will fix this myself.

@sjm41 sjm41 reopened this May 31, 2024
@sjm41 sjm41 closed this as completed in 90ff268 May 31, 2024
sjm41 added a commit that referenced this issue May 31, 2024
Removed ligase activity parent. Fixes #27928
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