fastq_extractor_proof_of_principle

this repo tries to optimize some Perl scripts which are part of the CRISPRAnalyzer R shiny package and which are too slow for production use in web applications. The original code's benchmark is as follow (please note: PERL script not included here)

all examples are done on Thinkpad T420s Core i7 vPro with 16GB RAM and Evo 850 SSD.

PERL

time perl CRISPR-extract.pl "ACC(.{20,21})G" ./data/TRAIL-Replicate1.fastq no

real	0m45.006s
user	0m44.191s
sys	0m0.682s

RUST

$ time fastq_parser ./data/TRAIL-Replicate1.fastq 

output

real	0m3.866s
user	0m3.414s
sys	0m0.438s

C

time ./extractor default ./data/TRAIL-Replicate1.fastq  no

output

real	0m4.409s
user	0m3.953s
sys	0m0.445s

TODO: make the regexp parsing multithreaded in RUST on big big input files

unbelievable the Rust code did beat the low-level C code, pretty amazing!

sam_mapper in RUST

PERL (not included in this repo)

time perl CRISPR-mapping.pl ./data/pilotscreen.fasta ./data/TRAIL-Replicate1_extracted.sam "M{20,21}$" "_"

output

real	1m17.280s
user	1m16.820s
sys	0m0.192s

RUST

time sam_mapper -f ./data/pilotscreen.fasta -s ./data/TRAIL-Replicate1_extracted.sam

output

real	0m7.590s
user	0m7.461s
sys	0m0.111s