The CRISPR/Cas system is a highly specific genome editing tool capable of distinguishing alleles differing by even a single base pair. Target sites might carry genetic variations that are not distinguishable by sgRNA designing tools based on one reference genome. AlleleAnalyzer is open-source software that incorporates single nucleotide variants and short insertions and deletions to design sgRNAs for precisely editing one or multiple haplotypes of a sequenced genome from any species, currently supporting eleven Cas proteins. It also leverages patterns of shared genetic variation to optimize sgRNA design for different populations. AlleleAnalyzer is available at https://github.com/keoughkath/AlleleAnalyzer.
Note: If you use this tool and find any issues or anything that is difficult to interpret, please send a message to this Github or to kathleen.keough at gladstone.ucsf.edu
If you use this tool, please cite our paper in Genome Biology.
Table of Contents
- Getting started
- Tool descriptions