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support files as input
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lh3 committed Jun 19, 2017
1 parent 10d176f commit 49ccb27
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Showing 8 changed files with 313 additions and 43 deletions.
75 changes: 51 additions & 24 deletions cli.c
@@ -1,7 +1,10 @@
#include <stdio.h>
#include <string.h>
#include <unistd.h>
#include <zlib.h>
#include "ksw2.h"
#include "kseq.h"
KSEQ_INIT(gzFile, gzread)

unsigned char seq_nt4_table[256] = {
0, 1, 2, 3, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4, 4,
Expand Down Expand Up @@ -36,43 +39,67 @@ static void ksw_gen_simple_mat(int m, int8_t *mat, int8_t a, int8_t b)
mat[(m - 1) * m + j] = 0;
}

static void global_aln(const char *algo, void *km, char *qseq, char *tseq, int8_t m, const int8_t *mat, int8_t q, int8_t e, int w, int zdrop, int flag, ksw_extz_t *ez)
{
int i, qlen, tlen;
qlen = strlen(qseq);
tlen = strlen(tseq);
for (i = 0; i < qlen; ++i)
qseq[i] = seq_nt4_table[(uint8_t)qseq[i]];
for (i = 0; i < tlen; ++i)
tseq[i] = seq_nt4_table[(uint8_t)tseq[i]];
if (w < 0) w = qlen > tlen? qlen : tlen;
if (strcmp(algo, "gg") == 0) ez->score = ksw_gg(0, qlen, (uint8_t*)qseq, tlen, (uint8_t*)tseq, 5, mat, q, e, w, &ez->m_cigar, &ez->n_cigar, &ez->cigar);
else if (strcmp(algo, "gg2") == 0) ez->score = ksw_gg2(0, qlen, (uint8_t*)qseq, tlen, (uint8_t*)tseq, 5, mat, q, e, w, &ez->m_cigar, &ez->n_cigar, &ez->cigar);
else if (strcmp(algo, "gg2_sse") == 0) ez->score = ksw_gg2_sse(0, qlen, (uint8_t*)qseq, tlen, (uint8_t*)tseq, 5, mat, q, e, w, &ez->m_cigar, &ez->n_cigar, &ez->cigar);
else if (strcmp(algo, "extz2_sse") == 0) ksw_extz2_sse(0, qlen, (uint8_t*)qseq, tlen, (uint8_t*)tseq, 5, mat, q, e, w, zdrop, flag, ez);
else abort();
}

int main(int argc, char *argv[])
{
int8_t a = 1, b = 1, q = 1, e = 1;
int c, i, qlen, tlen, n_cigar, score;
char *qseq, *tseq, *algo = "gg2";
uint32_t *cigar;
int c, i, pair = 1, w = -1;
char *algo = "gg2";
int8_t mat[25];
ksw_extz_t ez;
gzFile fp[2];

while ((c = getopt(argc, argv, "t:")) >= 0) {
while ((c = getopt(argc, argv, "t:w:")) >= 0) {
if (c == 't') algo = optarg;
else if (c == 'w') w = atoi(optarg);
}
if (argc - optind < 2) {
fprintf(stderr, "Usage: ksw2-global <DNA-target> <DNA-query>\n");
return 1;
}
memset(&ez, 0, sizeof(ksw_extz_t));
ksw_gen_simple_mat(5, mat, a, -b);
tseq = argv[optind], qseq = argv[optind+1];
tlen = strlen(tseq);
qlen = strlen(qseq);
for (i = 0; i < qlen; ++i)
qseq[i] = seq_nt4_table[(uint8_t)qseq[i]];
for (i = 0; i < tlen; ++i)
tseq[i] = seq_nt4_table[(uint8_t)tseq[i]];
if (strcmp(algo, "gg") == 0) score = ksw_gg(0, qlen, (uint8_t*)qseq, tlen, (uint8_t*)tseq, 5, mat, q, e, qlen > tlen? qlen : tlen, &n_cigar, &cigar);
else if (strcmp(algo, "gg2") == 0) score = ksw_gg2(0, qlen, (uint8_t*)qseq, tlen, (uint8_t*)tseq, 5, mat, q, e, qlen > tlen? qlen : tlen, &n_cigar, &cigar);
else if (strcmp(algo, "gg2_sse") == 0) score = ksw_gg2_sse(0, qlen, (uint8_t*)qseq, tlen, (uint8_t*)tseq, 5, mat, q, e, qlen > tlen? qlen : tlen, &n_cigar, &cigar);
else if (strcmp(algo, "extz_sse") == 0) {
ksw_extz_sse(0, qlen, (uint8_t*)qseq, tlen, (uint8_t*)tseq, 5, mat, q, e, qlen > tlen? qlen : tlen, 100, 0, &ez);
n_cigar = ez.n_cigar, cigar = ez.cigar, score = ez.score;
printf("max: %d; (%d,%d)\n", ez.max, ez.max_t, ez.max_q);
printf("mqe: %d; %d\n", ez.mqe, ez.mqe_t);
} else abort();
printf("%d\t", score);
for (i = 0; i < n_cigar; ++i)
printf("%d%c", cigar[i]>>4, "MID"[cigar[i]&0xf]);
printf("\n");
fp[0] = gzopen(argv[optind], "r");
fp[1] = gzopen(argv[optind+1], "r");

if (fp[0] == 0 && fp[1] == 0) {
global_aln(algo, 0, argv[optind+1], argv[optind], 5, mat, q, e, w, 100, 0, &ez);
printf("%d\t", ez.score);
for (i = 0; i < ez.n_cigar; ++i)
printf("%d%c", ez.cigar[i]>>4, "MID"[ez.cigar[i]&0xf]);
printf("\n");
} else if (fp[0] && fp[1]) {
kseq_t *ks[2];
ks[0] = kseq_init(fp[0]);
ks[1] = kseq_init(fp[1]);
if (pair) {
while (kseq_read(ks[0]) > 0) {
if (kseq_read(ks[1]) <= 0) break;
global_aln(algo, 0, ks[0]->seq.s, ks[1]->seq.s, 5, mat, q, e, w, 100, 0, &ez);
printf("%s\t%s\t%d\t", ks[0]->name.s, ks[1]->name.s, ez.score);
for (i = 0; i < ez.n_cigar; ++i)
printf("%d%c", ez.cigar[i]>>4, "MID"[ez.cigar[i]&0xf]);
printf("\n");
}
}
kseq_destroy(ks[0]);
kseq_destroy(ks[1]);
}
return 0;
}
242 changes: 242 additions & 0 deletions kseq.h
@@ -0,0 +1,242 @@
/* The MIT License
Copyright (c) 2008, 2009, 2011 Attractive Chaos <attractor@live.co.uk>
Permission is hereby granted, free of charge, to any person obtaining
a copy of this software and associated documentation files (the
"Software"), to deal in the Software without restriction, including
without limitation the rights to use, copy, modify, merge, publish,
distribute, sublicense, and/or sell copies of the Software, and to
permit persons to whom the Software is furnished to do so, subject to
the following conditions:
The above copyright notice and this permission notice shall be
included in all copies or substantial portions of the Software.
THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND,
EXPRESS OR IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF
MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE AND
NONINFRINGEMENT. IN NO EVENT SHALL THE AUTHORS OR COPYRIGHT HOLDERS
BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER LIABILITY, WHETHER IN AN
ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, OUT OF OR IN
CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN THE
SOFTWARE.
*/

/* Last Modified: 05MAR2012 */

#ifndef AC_KSEQ_H
#define AC_KSEQ_H

#include <ctype.h>
#include <string.h>
#include <stdlib.h>

#define KS_SEP_SPACE 0 // isspace(): \t, \n, \v, \f, \r
#define KS_SEP_TAB 1 // isspace() && !' '
#define KS_SEP_LINE 2 // line separator: "\n" (Unix) or "\r\n" (Windows)
#define KS_SEP_MAX 2

#define __KS_TYPE(type_t) \
typedef struct __kstream_t { \
unsigned char *buf; \
int begin, end, is_eof; \
type_t f; \
} kstream_t;

#define ks_err(ks) ((ks)->end == -1)
#define ks_eof(ks) ((ks)->is_eof && (ks)->begin >= (ks)->end)
#define ks_rewind(ks) ((ks)->is_eof = (ks)->begin = (ks)->end = 0)

#define __KS_BASIC(type_t, __bufsize) \
static inline kstream_t *ks_init(type_t f) \
{ \
kstream_t *ks = (kstream_t*)calloc(1, sizeof(kstream_t)); \
ks->f = f; \
ks->buf = (unsigned char*)malloc(__bufsize); \
return ks; \
} \
static inline void ks_destroy(kstream_t *ks) \
{ \
if (ks) { \
free(ks->buf); \
free(ks); \
} \
}

#define __KS_GETC(__read, __bufsize) \
static inline int ks_getc(kstream_t *ks) \
{ \
if (ks_err(ks)) return -3; \
if (ks->is_eof && ks->begin >= ks->end) return -1; \
if (ks->begin >= ks->end) { \
ks->begin = 0; \
ks->end = __read(ks->f, ks->buf, __bufsize); \
if (ks->end == 0) { ks->is_eof = 1; return -1;} \
if (ks->end == -1) { ks->is_eof = 1; return -3;}\
} \
return (int)ks->buf[ks->begin++]; \
}

#ifndef KSTRING_T
#define KSTRING_T kstring_t
typedef struct __kstring_t {
size_t l, m;
char *s;
} kstring_t;
#endif

#ifndef kroundup32
#define kroundup32(x) (--(x), (x)|=(x)>>1, (x)|=(x)>>2, (x)|=(x)>>4, (x)|=(x)>>8, (x)|=(x)>>16, ++(x))
#endif

#define __KS_GETUNTIL(__read, __bufsize) \
static int ks_getuntil2(kstream_t *ks, int delimiter, kstring_t *str, int *dret, int append) \
{ \
int gotany = 0; \
if (dret) *dret = 0; \
str->l = append? str->l : 0; \
for (;;) { \
int i; \
if (ks_err(ks)) return -3; \
if (ks->begin >= ks->end) { \
if (!ks->is_eof) { \
ks->begin = 0; \
ks->end = __read(ks->f, ks->buf, __bufsize); \
if (ks->end == 0) { ks->is_eof = 1; break; } \
if (ks->end == -1) { ks->is_eof = 1; return -3; } \
} else break; \
} \
if (delimiter == KS_SEP_LINE) { \
for (i = ks->begin; i < ks->end; ++i) \
if (ks->buf[i] == '\n') break; \
} else if (delimiter > KS_SEP_MAX) { \
for (i = ks->begin; i < ks->end; ++i) \
if (ks->buf[i] == delimiter) break; \
} else if (delimiter == KS_SEP_SPACE) { \
for (i = ks->begin; i < ks->end; ++i) \
if (isspace(ks->buf[i])) break; \
} else if (delimiter == KS_SEP_TAB) { \
for (i = ks->begin; i < ks->end; ++i) \
if (isspace(ks->buf[i]) && ks->buf[i] != ' ') break; \
} else i = 0; /* never come to here! */ \
if (str->m - str->l < (size_t)(i - ks->begin + 1)) { \
str->m = str->l + (i - ks->begin) + 1; \
kroundup32(str->m); \
str->s = (char*)realloc(str->s, str->m); \
} \
gotany = 1; \
memcpy(str->s + str->l, ks->buf + ks->begin, i - ks->begin); \
str->l = str->l + (i - ks->begin); \
ks->begin = i + 1; \
if (i < ks->end) { \
if (dret) *dret = ks->buf[i]; \
break; \
} \
} \
if (!gotany && ks_eof(ks)) return -1; \
if (str->s == 0) { \
str->m = 1; \
str->s = (char*)calloc(1, 1); \
} else if (delimiter == KS_SEP_LINE && str->l > 1 && str->s[str->l-1] == '\r') --str->l; \
str->s[str->l] = '\0'; \
return str->l; \
} \
static inline int ks_getuntil(kstream_t *ks, int delimiter, kstring_t *str, int *dret) \
{ return ks_getuntil2(ks, delimiter, str, dret, 0); }

#define KSTREAM_INIT(type_t, __read, __bufsize) \
__KS_TYPE(type_t) \
__KS_BASIC(type_t, __bufsize) \
__KS_GETC(__read, __bufsize) \
__KS_GETUNTIL(__read, __bufsize)

#define kseq_rewind(ks) ((ks)->last_char = (ks)->f->is_eof = (ks)->f->begin = (ks)->f->end = 0)

#define __KSEQ_BASIC(SCOPE, type_t) \
SCOPE kseq_t *kseq_init(type_t fd) \
{ \
kseq_t *s = (kseq_t*)calloc(1, sizeof(kseq_t)); \
s->f = ks_init(fd); \
return s; \
} \
SCOPE void kseq_destroy(kseq_t *ks) \
{ \
if (!ks) return; \
free(ks->name.s); free(ks->comment.s); free(ks->seq.s); free(ks->qual.s); \
ks_destroy(ks->f); \
free(ks); \
}

/* Return value:
>=0 length of the sequence (normal)
-1 end-of-file
-2 truncated quality string
-3 error reading stream
*/
#define __KSEQ_READ(SCOPE) \
SCOPE int kseq_read(kseq_t *seq) \
{ \
int c,r; \
kstream_t *ks = seq->f; \
if (seq->last_char == 0) { /* then jump to the next header line */ \
while ((c = ks_getc(ks)) >= 0 && c != '>' && c != '@'); \
if (c < 0) return c; /* end of file or error*/ \
seq->last_char = c; \
} /* else: the first header char has been read in the previous call */ \
seq->comment.l = seq->seq.l = seq->qual.l = 0; /* reset all members */ \
if ((r=ks_getuntil(ks, 0, &seq->name, &c)) < 0) return r; /* normal exit: EOF or error */ \
if (c != '\n') ks_getuntil(ks, KS_SEP_LINE, &seq->comment, 0); /* read FASTA/Q comment */ \
if (seq->seq.s == 0) { /* we can do this in the loop below, but that is slower */ \
seq->seq.m = 256; \
seq->seq.s = (char*)malloc(seq->seq.m); \
} \
while ((c = ks_getc(ks)) >= 0 && c != '>' && c != '+' && c != '@') { \
if (c == '\n') continue; /* skip empty lines */ \
seq->seq.s[seq->seq.l++] = c; /* this is safe: we always have enough space for 1 char */ \
ks_getuntil2(ks, KS_SEP_LINE, &seq->seq, 0, 1); /* read the rest of the line */ \
} \
if (c == '>' || c == '@') seq->last_char = c; /* the first header char has been read */ \
if (seq->seq.l + 1 >= seq->seq.m) { /* seq->seq.s[seq->seq.l] below may be out of boundary */ \
seq->seq.m = seq->seq.l + 2; \
kroundup32(seq->seq.m); /* rounded to the next closest 2^k */ \
seq->seq.s = (char*)realloc(seq->seq.s, seq->seq.m); \
} \
seq->seq.s[seq->seq.l] = 0; /* null terminated string */ \
if (c != '+') return seq->seq.l; /* FASTA */ \
if (seq->qual.m < seq->seq.m) { /* allocate memory for qual in case insufficient */ \
seq->qual.m = seq->seq.m; \
seq->qual.s = (char*)realloc(seq->qual.s, seq->qual.m); \
} \
while ((c = ks_getc(ks)) >= 0 && c != '\n'); /* skip the rest of '+' line */ \
if (c == -1) return -2; /* error: no quality string */ \
while ((c = ks_getuntil2(ks, KS_SEP_LINE, &seq->qual, 0, 1) >= 0 && seq->qual.l < seq->seq.l)); \
if (c == -3) return -3; /* stream error */ \
seq->last_char = 0; /* we have not come to the next header line */ \
if (seq->seq.l != seq->qual.l) return -2; /* error: qual string is of a different length */ \
return seq->seq.l; \
}

#define __KSEQ_TYPE(type_t) \
typedef struct { \
kstring_t name, comment, seq, qual; \
int last_char; \
kstream_t *f; \
} kseq_t;

#define KSEQ_INIT2(SCOPE, type_t, __read) \
KSTREAM_INIT(type_t, __read, 16384) \
__KSEQ_TYPE(type_t) \
__KSEQ_BASIC(SCOPE, type_t) \
__KSEQ_READ(SCOPE)

#define KSEQ_INIT(type_t, __read) KSEQ_INIT2(static, type_t, __read)

#define KSEQ_DECLARE(type_t) \
__KS_TYPE(type_t) \
__KSEQ_TYPE(type_t) \
extern kseq_t *kseq_init(type_t fd); \
void kseq_destroy(kseq_t *ks); \
int kseq_read(kseq_t *seq);

#endif
9 changes: 5 additions & 4 deletions ksw2.h
Expand Up @@ -38,20 +38,21 @@ extern "C" {
* @param flag flag (see KSW_EZ_* macros)
* @param ez (out) scores and cigar
*/
void ksw_extz_sse(void *km, int qlen, const uint8_t *query, int tlen, const uint8_t *target, int8_t m, const int8_t *mat, int8_t q, int8_t e, int w, int zdrop, int flag, ksw_extz_t *ez);
void ksw_extz2_sse(void *km, int qlen, const uint8_t *query, int tlen, const uint8_t *target, int8_t m, const int8_t *mat, int8_t q, int8_t e, int w, int zdrop, int flag, ksw_extz_t *ez);

/**
* Global alignment
*
* (first 10 parameters identical to ksw_extz_sse())
* @param m_cigar (modified) max CIGAR length; feed 0 if cigar==0
* @param n_cigar (out) number of CIGAR elements
* @param cigar (out) BAM-encoded CIGAR; caller need to deallocate with kfree(km, )
*
* @return score of the alignment
*/
int ksw_gg(void *km, int qlen, const uint8_t *query, int tlen, const uint8_t *target, int8_t m, const int8_t *mat, int8_t gapo, int8_t gape, int w, int *n_cigar_, uint32_t **cigar_);
int ksw_gg2(void *km, int qlen, const uint8_t *query, int tlen, const uint8_t *target, int8_t m, const int8_t *mat, int8_t gapo, int8_t gape, int w, int *n_cigar_, uint32_t **cigar_);
int ksw_gg2_sse(void *km, int qlen, const uint8_t *query, int tlen, const uint8_t *target, int8_t m, const int8_t *mat, int8_t gapo, int8_t gape, int w, int *n_cigar_, uint32_t **cigar_);
int ksw_gg(void *km, int qlen, const uint8_t *query, int tlen, const uint8_t *target, int8_t m, const int8_t *mat, int8_t gapo, int8_t gape, int w, int *m_cigar_, int *n_cigar_, uint32_t **cigar_);
int ksw_gg2(void *km, int qlen, const uint8_t *query, int tlen, const uint8_t *target, int8_t m, const int8_t *mat, int8_t gapo, int8_t gape, int w, int *m_cigar_, int *n_cigar_, uint32_t **cigar_);
int ksw_gg2_sse(void *km, int qlen, const uint8_t *query, int tlen, const uint8_t *target, int8_t m, const int8_t *mat, int8_t gapo, int8_t gape, int w, int *m_cigar_, int *n_cigar_, uint32_t **cigar_);

#ifdef __cplusplus
}
Expand Down
8 changes: 4 additions & 4 deletions ksw2_ext.c
Expand Up @@ -20,9 +20,9 @@ int ksw_ext(void *km, int qlen, const uint8_t *query, int tlen, const uint8_t *t
}

// fill the first row
eh[0].h = h0, eh[0].e = h0 - gapoe - gapo > 0? h0 - gapoe - gapo : 0;
eh[0].h = h0, eh[0].e = h0 - gapoe - gapoe > 0? h0 - gapoe - gapoe : 0;
for (j = 1; j <= qlen && j <= w; ++j) {
eh[j].h = h0 - (gapo + gape * j), eh[j].e = h0 - (gapoe + gapo + gape * j);
eh[j].h = h0 - (gapoe + gape * j), eh[j].e = h0 - (gapoe + gapoe + gape * j);
if (eh[j].e < 0) eh[j].e = 0;
if (eh[j].h < 0) {
eh[j].h = 0;
Expand All @@ -49,9 +49,9 @@ int ksw_ext(void *km, int qlen, const uint8_t *query, int tlen, const uint8_t *t
if (en > max_j0 + w + 1) en = max_j0 + w + 1;
// compute the first column
if (st == 0) {
h1 = h0 - (gapo + gape * i);
h1 = h0 - (gapoe + gape * i);
if (h1 < 0) h1 = 0;
f = h0 - (gapoe + gapo + gape * i);
f = h0 - (gapoe + gapoe + gape * i);
if (f < 0) f = 0;
} else h1 = 0;
for (j = st; j < en; ++j) {
Expand Down

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