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Update README_for_non_human_genome.md
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likelet committed Jul 10, 2018
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Expand Up @@ -54,9 +54,10 @@ sample2_rep2_2.fq.gz

* `Design.file` are required if you are going to perform differential expression analysis between groups.

###Step 2. Edit `nextflow.config` file
### Step 2. Edit `nextflow.config` file
Leave the other line unchanged, modified the following sentences like below (According to the location of download files):
> If your are using docker in your server, plz modify `docker.config` instead.
> If your are using docker in your server, plz modify `docker.config` instead.
```shell
fastq_ext = '*_{1,2}.fq.gz'
fasta_ref = '~/database/mouse/GRCm38.p5.genome.fa'
Expand All @@ -71,7 +72,8 @@ Leave the other line unchanged, modified the following sentences like below (Acc
known_lncRNA_gtf="gencode.vM16.annotation.gtf"

```
###Step 3. Start your analysis trip with command below
### Step 3. Start your analysis trip with command below

```shell
nextflow run -with-trace -with-report report.html -with-timeline timeline.html LncRNAanalysisPipe.nf
#or running in a docker image
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