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Primer design not working #33
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Can you send me your sequence file to me for debug. |
Dear Dr. Lianming Du,
Please find the attached file (3 GB in size), Kindly let me know once you
download it, i will remove it from my drive space.
I am able to identify SSR but not able to design primers, it will be great
if you can help.
cdat_masurca_scf.tar.xz
<https://drive.google.com/file/d/1YcA4q9Xb7OrizNykK-G629OzTSITqaiJ/view?usp=drive_web>
Regards
*Sheetal Ambardar*
Assistant Professor
School of Biotechnology
University of Jammu
Jammu & Kashmir 180006
…On Mon, 11 Sept 2023 at 09:35, Lianming Du ***@***.***> wrote:
Can you send me your sequence file to me for debug.
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Thanks! I have downloaded it. |
I have test Krait v1.5.1 using your data, it works well. You can delete the generated index file with extension of .fxi. And then redo search SSRs. |
Dear Dr. Lianming Du,
Thank you for analysing my sequences using krait. Were you able to design
primers too.
Regards
*Sheetal Ambardar*
Assistant Professor
School of Biotechnology
University of Jammu
Jammu & Kashmir 180006
…On Mon, 11 Sept 2023 at 16:20, Lianming Du ***@***.***> wrote:
I have test Krait using your data, it works well. You can delete the
generated index file with extension of .fxi. And then redo search SSRs.
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Yes. Use Krait v1.5.1, and then delete the index file with the same name of sequence file with suffix of .fxi. |
Thank you so much. It did worked with new version. |
Thank you for troubleshooting this error. |
SSR markers are identified but primer designing not working. Everytime you select any SSR marker, it shows the error
Here jcf7180029229335 is contig name
Traceback (most recent call last):
File "workers.py", line 113, in run
File "workers.py", line 457, in process
KeyError: 'jcf7180029229335 does not exist in fasta file'
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