Program : demultiplex.py
Description: determine the level of index hopping and barcode errors using the demultiplexing process
Parameters : none
Returned : 1. read quality figures and statistics 2. demultiplexed FASTQ files
Vocab
BARCODES - the unique sequences that were attached to your each invidivual samples’ genetic material before the samples got all mixed together. https://astrobiomike.github.io/amplicon/demultiplexing
DEMULTIPLEXING - refers to the step in processing where you’d use the barcode information in order to know which sequences came from which samples after they had all be sequenced together. https://astrobiomike.github.io/amplicon/demultiplexing
INDEX SWAPPING - a specific type of misassignment that results in the incorrect assignment of libraries from the expected index to a different index (in the multiplexed pool). https://www.illumina.com/science/education/minimizing-index-hopping.html