The expression level in DAP15 kernel of the 368 association panel was quantified based on RNA-seq. Read counts for each gene were calculated and scaled according to RPKM. After RPKM normalization, all genes with a median expression level larger than zero for each sample were included, and the overall distribution among 368 lines of expression levels for each gene is normalized using a normal quantile transformation.
Fu et al. RNA sequencing reveals the complex regulatory network in the maize kernel. Nat Commun. 2013; 4:2832. doi: 10.1038/ncomms3832.