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Feature "Spot intensity" calculation error #143
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Hello @drchrisch Not super recently this is suspect. Can share the image and the files? |
Sharing image is a bit difficult, I could send a link to the mastodon file. |
Ok I will try to reproduce. Thanks for the procedure. |
Hello @drchrisch |
Well, 200 MB is not that small. Sorry, silly question, but how does this FEX works and how do I restrict access to the data? |
Email me please.
…On Thu, Feb 10, 2022, 18:44 drchrisch ***@***.***> wrote:
Well, 200 MB is not that small. Sorry, silly question, but how does this
FEX works and how do I restrict access to the data?
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@drchrisch Thank you for the data. |
That's really weird, but matches expectation. I had this problem on two computers (Windows) with Fiji.app/jars/mastodon-1.0.0-beta-20.jar. |
Ok we run the same. Can you give me the parameters with which you run the detection and linking steps? |
DoG (diameter 4.0, threshold 100), simple LAP (15, 15, 2) (there should be a *.mastodon file as well?). |
Yes. And the mastodon file does not give an error :) |
Yes I got the bug! On the 2nd channel results. |
Cool! It's all in the data, right? |
Could you try to replace the core mastodon |
Great, looks conving. Many thanks! |
So the bug is fixed? If yes I will do a release but not immediately. |
Yes, solved. That's it for me and I will close the issue. |
Spot intensity calculation error
I analyze a two-channel dataset (20 timepoints, 3D images 20x1024x1024) with Mastodon (Fiji v1.53o, everything updated). It works fine to extract spots and links. But when I run "compute features" I get a java error for feature "Spot intensity" and only for this feature.
I did analyze a few other dataset earlier whithout getting this error. Were there some changes in the feature calculation part recently?
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