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Update quickstart.rst
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dbolotin committed Feb 14, 2018
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Expand Up @@ -177,7 +177,7 @@ For the full length cDNA-based immunoglobulin repertoire analysis we generally r
::
> mixcr align -p kaligner2 -s hsa -r alignmentReport.txt -OreadsLayout=Collinear \
-OvParameters.geneFeatureToAlign=VTranscript read_R1.fastq.gz read_R2.fastq.gz \
-OvParameters.geneFeatureToAlign=VTranscriptWithP read_R1.fastq.gz read_R2.fastq.gz \
alignments.vdjca
Option ``-s`` allows to specify species (e.g. homo sapiens - ``hsa``, mus musculus - ``mmu``). Parameter ``-OreadsLayout`` allow us to set paired-end reads orientation (``Collinear``, ``Opposite``, ``Unknown``). Note, that after MiGEC analysis paired-end read pairs are in ``Collinear`` orientation.
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