MoBIE project with two clem example datasets.
On-section CLEM data according to the protocol published in Correlated fluorescence and 3D electron microscopy with high sensitivity and spatial precision. Cells are HeLa_Kyoto_H2B - mCherry and GalT - GFP cell line as published in CellCognition: time-resolved phenotype annotation in high-throughput live cell imaging. The data was acquired and shared by Giulia Mizzon from the Electron Microscopy Core Facility at EMBL Heidelberg.
The dataset hela contains the following image sources:
- EM-overview Single EM overview stitched montage.
- em-detail, stitched montages covering individual grid squares
- fluorescence-overview: GFP fluorescence channel registered to the em
- low magnitude tomograms: EM tomograms at 5 nm isotropic resolution
- high magnitude tomograms: EM tomograms at 1.5 nm isotropic resolution
On-section CLEM data according to the protocol published in Correlated fluorescence and 3D electron microscopy with high sensitivity and spatial precision. From the publication Seipin and Nem1 establish discrete ER subdomains to initiate yeast lipid droplet biogenesis. The data was acquired and shared by Giulia Mizzon from the Electron Microscopy Core Facility at EMBL Heidelberg.
The dataset yeast contains the following image sources:
- EM-overview Single EM overview slices
- fluorescence-overview: GFP fluorescence channel registered to the em
- low magnitude tomograms: EM tomograms at 5 nm isotropic resolution
- high magnitude tomograms: EM tomograms at 1.25 nm isotropic resolution