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Added info about output to man page
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apmasell committed Mar 16, 2012
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Expand Up @@ -107,6 +107,35 @@ Input files will have their quality encoded as PHRED + 64 instead of the PHRED +
.TP
\-F
Normally, output will be as a FASTA even though per-base quality information is available. To retain this quality information, this flag will output the sequence and the quality information in FASTQ format with quality scores encoded as PHRED + 33. The meaning of the quality score is conceptually different from the input quality scores for the overlap region, but this may not matter depending on your downstream application.
.SH OUTPUT STATISTICS
At the end of reconstruction, several statistics are output on lines beginning with \fBSTAT\fR.
.TP
READS
The number of reads in the input files.
.TP
NOALGN
The number of sequences where there exists no overlap with a probability above the threshold.
.TP
NOFP
The number of sequences where the forward primer could not be aligned. This is only done when \fB-p\fR is supplied and a nucleotide sequence.
.TP
NORP
The number of sequences where the reverse primer could not be aligned. This is only done when \fB-q\fR is supplied and a nucleotide sequence.
.TP
LOWQ
The number of sequences where the quality score of the reconstruction (not the quality scores of the individual bases) is below the threshold.
.TP
DEGENERATE
The number of sequences containing uncalled/degenerate/N bases in the final reconstruction (it is immaterial if there are uncalled bases in the reads.) This is only done when \fB-N\fR is provided.
.TP
SHORT
The number of sequences where the final reconstructed sequence is too short. This is only done when \fB-l\fR is provided.
.TP
LONG
The number of sequences where the final reconstructed sequence is too long. This is only done when \fB-L\fR is provided.
.TP
OK
The number of sequences output.
.SH EXAMPLES
This will assemble a data from a run in lane 7:

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