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Sample_name,Organism_name1,Organism_name2,Genome1_size,Genome2_size,nb_aDNA_bp_aligned_genome1,nb_aDNA_bp_aligned_genome2,NormalizedReadRatio | ||
metagenome,Bacillus_subtilis,Escherichia_coli,4215606,4686137,1453802,9104,7.471773618852114 | ||
metagenomebis,Bacillus_subtilis,Escherichia_coli,4215606,4686137,4855,3598032,-9.380861910144272 |
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Output | ||
====== | ||
# Output | ||
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## coproID results | ||
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- `results/{fastq_file_basename}.html` | ||
- `results/coproID_result.html` | ||
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This file is the report of **coproID** and contains explanations on how the calculation were performed, the files used, and your results. | ||
This file is the report of **coproID** and contains explanations on how the calculation were performed, the files used, and your results. | ||
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An example **coproID** output file file can be found [here](_static/coproID_result.html) | ||
An example **coproID** output file file can be found [here](_static/coproID_result.html) | ||
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- `results/multiqc_report.html` | ||
- `results/coproid_result.csv` | ||
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This file is generated by [MultiQC](http://multiqc.info/) and reports the metrics of the different tools used by **coproID** | ||
This file contains the numerical results of **coproID** | ||
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An example **coproID** MultiQC report can be found [here](_static/multiqc_report.html) | ||
An example **coproID** output file file can be found [here](_static/coproid_result.csv) | ||
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- `results/mapdamage_{target_organism}/*` | ||
- `results/multiqc_report.html` | ||
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[MapDamage ](https://ginolhac.github.io/mapDamage/) plots. These plots are included in the **coproID** reports. | ||
This file is generated by [MultiQC](http://multiqc.info/) and reports the metrics of the different tools used by **coproID** | ||
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- `results/bam/{target_organism}/*bam` | ||
An example **coproID** MultiQC report can be found [here](_static/multiqc_report.html) | ||
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The filtered alignment `bam` files on each target genome | ||
- `results/mapdamage_{target_organism}/*` | ||
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[MapDamage ](https://ginolhac.github.io/mapDamage/) plots. These plots are included in the **coproID** reports. | ||
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- `results/bam/{target_organism}/*bam` | ||
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The filtered alignment `bam` files on each target genome | ||
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## coproID logs | ||
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You can generate a log file report of coproID run by using nextflow reporting option `-with-report` | ||
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Example: | ||
``` | ||
nextflow run maxibor/coproid --genome1 'path/to/data/genomes/hsapiens.fa' --genome2 'path/to/data/genomes/cfamiliaris.fa' --name1 'data/genomes/Homo_sapiens' --name2 'Canis_familiaris' --reads '*_R{1,2}.fastq.gz' -with-report report.html | ||
``` | ||
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Here the log file will be saved as `report.html` | ||
nextflow run maxibor/coproid --genome1 'path/to/data/genomes/hsapiens.fa' --genome2 'path/to/data/genomes/cfamiliaris.fa' --name1 'data/genomes/Homo_sapiens' --name2 'Canis_familiaris' --reads '*_R{1,2}.fastq.gz' -with-report report.html | ||
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This log file will be saved as `report.html` |
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Troubleshooting | ||
=============== | ||
# Troubleshooting | ||
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## Known issues | ||
- On Mac OS X, the nextflow log reporting (`-with-report`) may not work with Nextflow v0.31.1. This should be fixed in future Nextflow releases. | ||
- If the DNA damage levels are too low, mapDamage will crash. | ||
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- On Mac OS X, the nextflow log reporting (`-with-report`) may not work with Nextflow v0.31.1. This should be fixed in future Nextflow releases. | ||
- If the DNA damage levels are too low, mapDamage may crash. | ||
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## Other issues | ||
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If you find a specific issue, please open an [issue](https://github.com/maxibor/coproid/issues) on [GitHub](https://github.com/maxibor/coproid/issues) |
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