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Update genome blacklists to actual CUT&RUN data #208

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chris-cheshire opened this issue Aug 31, 2023 · 0 comments
Closed

Update genome blacklists to actual CUT&RUN data #208

chris-cheshire opened this issue Aug 31, 2023 · 0 comments
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enhancement Improvement for existing functionality

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@chris-cheshire
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Description of feature

https://doi.org/10.1186/s13059-023-03027-3

The CUT&RUN suspect list of problematic regions of the genome - Genome Biology
Background Cleavage Under Targets and Release Using Nuclease (CUT&RUN) is an increasingly popular technique to map genome-wide binding profiles of histone modifications, transcription factors, and co-factors. The ENCODE project and others have compiled blacklists for ChIP-seq which have been widely adopted: these lists contain regions of high and unstructured signal, regardless of cell type or protein target, indicating that these are false positives. While CUT&RUN obtains similar results to ChIP-seq, its biochemistry and subsequent data analyses are different. We found that this results in a CUT&RUN-specific set of undesired high-signal regions.

@chris-cheshire chris-cheshire added the enhancement Improvement for existing functionality label Aug 31, 2023
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