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Hello, I'm the beginner of the nf-core framework.
In my experiment, there is no spike-in control. But I want to use the cutandrun to analysis my data. Found no option to skip the spike-in in pipeline.
The text was updated successfully, but these errors were encountered:
Hi, thanks for your message. The default spike-in is the ecoli DNA that is a natural by-product of mnase or Tn5 production. Did you explicitly remove the spike-in from your samples? If not, then you will have ecoli DNA in your samples that the pipeline will use. If you did explicitly remove this DNA and you did not spike it back in, then this is something currently unsupported in the pipeline as it Is not a recommended way to perform CUT&RUN experiments.
Hello, I'm the beginner of the nf-core framework.
In my experiment, there is no spike-in control. But I want to use the cutandrun to analysis my data. Found no option to skip the spike-in in pipeline.
The text was updated successfully, but these errors were encountered: