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Separate cell barcode counting and filtering #126

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olgabot opened this issue Jan 6, 2021 · 0 comments
Open

Separate cell barcode counting and filtering #126

olgabot opened this issue Jan 6, 2021 · 0 comments
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enhancement New feature or request

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@olgabot
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olgabot commented Jan 6, 2021

Is your feature request related to a problem? Please describe

Currently, if --tenxt_min_umi_per_cell is changed, then count_umi_per_cell restarts from zero, even though all the UMIs are already counted, it's the filtering that happens separately. The count_umi_per_cell process takes ~5-10 hours to complete per bam file, so it would be ideal to not have to rerun it.

Describe the solution you'd like

Set the min umi per cell in count_umi_per_cell to 0, then separately use Nextflow to filter the per-barcode UMI counts using the params.tenx_min_umi_per_cell value.

Describe alternatives you've considered

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@olgabot olgabot added the enhancement New feature or request label Jan 6, 2021
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