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add module for mcroni #1750

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Jun 13, 2022
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43 changes: 43 additions & 0 deletions modules/mcroni/main.nf
Original file line number Diff line number Diff line change
@@ -0,0 +1,43 @@
def VERSION = '1.0.4' // Version information not provided by tool on CLI

process MCRONI {
tag "$meta.id"
label 'process_low'

conda (params.enable_conda ? "bioconda::mcroni=1.0.4" : null)
container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
'https://depot.galaxyproject.org/singularity/mcroni%3A1.0.4--pyh5e36f6f_0':
'quay.io/biocontainers/mcroni:1.0.4--pyh5e36f6f_0' }"

input:
tuple val(meta), path(fasta)

output:
tuple val(meta), path("*.tsv") , emit: tsv
tuple val(meta), path("*.fa"), optional: true, emit: fa
path "versions.yml" , emit: versions

when:
task.ext.when == null || task.ext.when

script:
def args = task.ext.args ?: ''
def prefix = task.ext.prefix ?: "${meta.id}"
def is_compressed = fasta.getName().endsWith(".gz") ? true : false
def fasta_name = fasta.getName().replace(".gz", "")
"""
if [ "$is_compressed" == "true" ]; then
gzip -c -d $fasta > $fasta_name
fi

mcroni \\
$args \\
--output $prefix \\
--fasta $fasta_name

cat <<-END_VERSIONS > versions.yml
"${task.process}":
mcroni: $VERSION
END_VERSIONS
"""
}
46 changes: 46 additions & 0 deletions modules/mcroni/meta.yml
Original file line number Diff line number Diff line change
@@ -0,0 +1,46 @@
name: "mcroni"
description: Analysis of mcr-1 gene (mobilized colistin resistance) for sequence variation
keywords:
- resistance
- fasta
tools:
- "mcroni":
description: "Scripts for finding and processing promoter variants upstream of mcr-1"
homepage: "https://github.com/liampshaw/mcroni"
documentation: "https://github.com/liampshaw/mcroni"
tool_dev_url: "https://github.com/liampshaw/mcroni"
doi: ""
licence: "['MIT']"

input:
- meta:
type: map
description: |
Groovy Map containing sample information
e.g. [ id:'test', single_end:false ]
- fasta:
type: file
description: A fasta file.
pattern: "*.{fasta.gz,fasta,fa.gz,fa,fna.gz,fna}"

output:
- meta:
type: map
description: |
Groovy Map containing sample information
e.g. [ id:'test', single_end:false ]
- versions:
type: file
description: File containing software versions
pattern: "versions.yml"
- tsv:
type: file
description: mcroni results in TSV format
pattern: "*.tsv"
- fa:
type: file
description: mcr-1 matching sequences
pattern: "*.fa"

authors:
- "@rpetit3"
4 changes: 4 additions & 0 deletions tests/config/pytest_modules.yml
Original file line number Diff line number Diff line change
Expand Up @@ -1278,6 +1278,10 @@ maxquant/lfq:
- modules/maxquant/lfq/**
- tests/modules/maxquant/lfq/**

mcroni:
- modules/mcroni/**
- tests/modules/mcroni/**

md5sum:
- modules/md5sum/**
- tests/modules/md5sum/**
Expand Down
13 changes: 13 additions & 0 deletions tests/modules/mcroni/main.nf
Original file line number Diff line number Diff line change
@@ -0,0 +1,13 @@
#!/usr/bin/env nextflow

nextflow.enable.dsl = 2

include { MCRONI } from '../../../modules/mcroni/main.nf'

workflow test_mcroni {

input = [ [ id:'test', single_end:false ], // meta map
file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true) ]

MCRONI ( input )
}
5 changes: 5 additions & 0 deletions tests/modules/mcroni/nextflow.config
Original file line number Diff line number Diff line change
@@ -0,0 +1,5 @@
process {

publishDir = { "${params.outdir}/${task.process.tokenize(':')[-1].tokenize('_')[0].toLowerCase()}" }

}
7 changes: 7 additions & 0 deletions tests/modules/mcroni/test.yml
Original file line number Diff line number Diff line change
@@ -0,0 +1,7 @@
- name: mcroni test_mcroni
command: nextflow run ./tests/modules/mcroni -entry test_mcroni -c ./tests/config/nextflow.config -c ./tests/modules/mcroni/nextflow.config
tags:
- mcroni
files:
- path: output/mcroni/test_table.tsv
md5sum: 64f8438dcc476e8b4d762fedc2e3f69e