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Hi Edmund,
I am raising a ticket here for the strand-flipping issue I mentioned on slack.
Bug:
BigWigs generated for PROseq samples appear to be flipped. The strand carrying the gene body signal is inconsistent with gene direction.
Example: GUSB gene on the negative strand. Genebody signal in the public Jurkat sample processed with the v2.1.1 pipeline is on the positive strand (UCSC Browser view attached).
The same issue was observed in internal PROseq samples as well.
Description of the bug
Hi Edmund,
I am raising a ticket here for the strand-flipping issue I mentioned on slack.
Bug:
BigWigs generated for PROseq samples appear to be flipped. The strand carrying the gene body signal is inconsistent with gene direction.
Example: GUSB gene on the negative strand. Genebody signal in the public Jurkat sample processed with the v2.1.1 pipeline is on the positive strand (UCSC Browser view attached).
The same issue was observed in internal PROseq samples as well.
Command used and terminal output
Relevant files
System information
Nextflow version: 23.04.1
Hardware : AWS Cloud9
Executor : Local
Container engine : Docker
OS:
Nascent pipeline version: v2.1.1-g9ff33c7
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