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ctb committed Oct 12, 2016
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21 changes: 19 additions & 2 deletions assemble.rst
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Expand Up @@ -17,9 +17,13 @@ Now, download some data::
curl -O https://s3-us-west-1.amazonaws.com/dib-training.ucdavis.edu/metagenomics-scripps-2016-10-12/SRR1976948.abundtrim.subset.pe.fq.gz
curl -O https://s3-us-west-1.amazonaws.com/dib-training.ucdavis.edu/metagenomics-scripps-2016-10-12/SRR1977249.abundtrim.subset.pe.fq.gz

These are data that have been run through the @@trimming.
These are data that have been run through k-mer abundance trimming
(see :doc:`kmer_trimming`) and subsampled so that we can run an assembly
in a fairly short time period :).

And, finally, run the assembler! ::
----

Now, finally, run the assembler! ::

mkdir /mnt/assembly
cd /mnt/assembly
Expand Down Expand Up @@ -62,6 +66,19 @@ MiSeq, and PacBio?
What kind of experimental design considerations should you have if you
plan to assemble?

Some figures: the first two come from work by Dr. Sherine Awad on
analyzing the data from Shakya et al (2014). The third comes from
an analysis of read search vs contig search of a protein database.

.. thumbnail:: files/assembler-runtimes.png
:width: 20%

.. thumbnail:: files/assembler-mapping.png
:width: 20%

.. thumbnail:: files/read-vs-contig-alignment.png
:width: 20%


After the assembly is finished
------------------------------
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