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Introduction to Computational Flow Cytometry in R

The goal of this repository is to provide the course materials that is used for the Introduction to Computational Flow Cytometry in R seminar. It includes the required code and presentation slides. It requires R version 4.3.0+ as well as Rstudio and the installation.R script can be used for installation of the required R packages.

Instructors: Gabriel Rosenfeld and Yuyan Yi

Contact: bioinformatics@niaid.nih.gov

Installation and Set up

After installing R and Rstudio, open the Rstudio and open the computational.flow.cytometry.r.Rproj file. This should open the project in Rstudio. In the console window, execute the following code: source("installation.R")

Useful Resources

Introductory Materials

Topics for further exploration

  • Normalization and Batch correction: cyCombine
  • Clustering and Differential Expression: diffcyt

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