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A simple Shiny app to deconvolute luciferase reporter data.

Implements the idea from Brown 2008 - Inferring gene expression dynamics from reporter protein levels - Biotechnol J . 2008 Nov;3(11):1437-48. doi: 10.1002/biot.200800133.

Can also calculates initial rate, time to maximum and decay rate of the deconvoluted trace.

Requirements

R, shiny, shinydashboard, ggplot2, doremi

Usage

Requires a CSV file as input formatted with two columns called "Time" and "Luminescence"

Input the halflife of your reporter in the same time units as time.

Press "Deconvolute". You're done!

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A simple Shiny app to deconvolute luciferase assay data

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