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EM-scripts

Various jiffies that I find useful for data processing.

convert_to_jpg uses ImageMagick to make a nice-looking jpg from an mrc.

project_map makes several projections of an input map in selected orientations using relion_project, and generates a stack of these projections for use as particle-picking templates.

rename_files renames a set of files to mic_00001.mrc, mic_00002.mrc, etc.

star2jpg takes a relion-generated data.star as input and uses ImageMagick to create jpgs of each mic with particle positions and numbers indicated, and per-mic Pmax, NSS and defocus printed - useful for post-refinement screening of mics, or as a sanity check to assess junkiness of 2D/3D classes.

unblur_gctf_wrapper runs unblur and Gctf, and creates contrast-adjusted jpg images with defocus etc written for diagnosis/screening. Requires ImageMagick. You may need to edit the names of the unblur, summovie and gctf executables. Run on multiple cores using GNU parallel, e.g.: find ./ -maxdepth 1 -name "mic*mrc" -print | parallel -j 12 './unblur.com {/}' >& log &

gnuplot_fsc makes a pretty-ish FSC curve in SVG format from relion_postprocess star file using gnuplot.

ice_catcher tries to guess whether an image has crystalline ice. Input is _avrot.txt files from CTFFIND.

gold_or_no_gold tries to guess whether an image has gold/carbon/vaccum present in the frame based on whether the histogram is bimodal or not.

run_ctffind runs ctffind on movies and generates some diagnostics (e.g. ice_catcher is integrated)

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