SPR evaluation of WYH compounds
Latest update posted on Issue #96
Yuhang Wang synthesised compounds were tested for binding with PaMurC, PaMurD and PaMurE using SPR (Biacore T200).
The experiments were run with a running buffer containing 20 mM HEPES pH 7.5, 200 mM NaCl, 10 mM MgCl2, and 5% DMSO. Proteins PaMurC/D/E were immobilised in an NTA sensor using His6-tagged proteins and amine coupling. For the dose response experiments, the starting compound concentrations were 500 uM (except 125 uM for WYH22-3-P).
The results should be fully validated with activity assays.
This is a summary table of the results:
nsb = none specific binding detected. (*) = There is nsb at higher concentrations. This value is likely to be overstimated.
For easier comparison, the structures are shown below:
Kinase Paper Link
AZ Paper Link
The graphs can be downloaded here: WYH_murC.docx WYH_murD.docx WYH_murE.docx