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Hi,
I have some problems with isoseq collapse. While most of my reads (90%) are mapped, almost half of them are filtered out after isoseq collpase. I was wondering how do you calculate coverage and identify? I am using the mg tag to get the identity, and calculating coverage per read as number of matches and mismatches in the cigar string divided by the read length, but I get much less reads filtered out than by isoseq collapse with the same thresholds. Are either coverage or identity calculated differently?
Many thanks
Mengjun
The text was updated successfully, but these errors were encountered:
Optional: If using single-cell workflow, read must be marked as coming from a real cell using the rc tag.
These minimum values can be changed using the following options.
Alignment Filter Options:
--min-aln-coverage FLOAT Ignore alignments with less than minimum query read coverage. [0.99]
--min-aln-identity FLOAT Ignore alignments with less than minimum alignment identity. [0.95]
Hi,
I have some problems with isoseq collapse. While most of my reads (90%) are mapped, almost half of them are filtered out after isoseq collpase. I was wondering how do you calculate coverage and identify? I am using the mg tag to get the identity, and calculating coverage per read as number of matches and mismatches in the cigar string divided by the read length, but I get much less reads filtered out than by isoseq collapse with the same thresholds. Are either coverage or identity calculated differently?
Many thanks
Mengjun
The text was updated successfully, but these errors were encountered: