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rpique committed Feb 4, 2017
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# QuASAR-MPRA: Accurate allele-specific analysis for massively parallel reporter assays
We have further developed our method for allele specific analysis QuASAR (quantitative allele-specific analysis of reads) ([Harvey et al, 2015]) to analyze allele specific signals in barcoded read counts data from MPRAs. Using this approach, we can take into account the uncertainty on the original plasmid proportions, over-dispersion, and sequencing errors. Here, we demonstrate how to use QuASAR-MPRA to analyze the MPRA data by [Tewhey et al,2016].
We have further developed our method for allele specific analysis QuASAR (quantitative allele-specific analysis of reads) ([Harvey et al, 2015]) to analyze allele specific signals in barcoded read counts data from MPRAs (preprint available in [Kalita et al,2017]). Using this approach, we can take into account the uncertainty on the original plasmid proportions, over-dispersion, and sequencing errors. Here, we demonstrate how to use QuASAR-MPRA to analyze the MPRA data by [Tewhey et al,2016].

The current software is still in development and we will kindly appreciate any comments and bug reports. We assume that you already have installed the QuASAR library.

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<!-- links -->
[Kalita et al,2017]:http://biorxiv.org/content/early/2017/02/03/105627
[Harvey et al, 2015]:http://bioinformatics.oxfordjournals.org/content/31/8/1235
[mpra.R]:mpra.R
[process.R]:process.R
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