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@untergasser untergasser released this Aug 17, 2019

release 2.5.0 - 2019-08-17

This release adds new features to primer3_core and related C code.

The primer3_config files for thermodynamic calculations are embedded in the
code and the primer3_config/ folder is not required any more for default
settings.

If PRIMER_SECONDARY_STRUCTURE_ALIGNMENT=1 is set, the secondary structure
will be displayed as string representation (for example):

PRIMER_LEFT_0_SELF_ANY_STUCT=5' ATTCGATTCTCCCCGGTATC 3'\n ||||\n 3' CTATGGCCCCTCTTAGCTTA 5'\n

for the structure:

5' ATTCGATTCTCCCCGGTATC 3'
               ||||
        3' CTATGGCCCCTCTTAGCTTA 5'

Minor fixes:

  1. Primer3 can handle windows newlines on input files.

  2. A bug in the compl_end calculations was fixed.

  3. The primer3_config/ folder is not required any more.

Assets 3