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@github-actions github-actions released this 29 Sep 13:56
· 1 commit to master since this release

This release of guidescan fixes a minor bug when using guidescan enumerate with options --format csv --mode complete.

In case no matches and no offtargets of the input gRNA were found, the order of the last 4 output csv fields would have been incorrect. This is fixed now.

This release also generates csv files if gRNA sequences are not exactly 23 NUCs long, instead of crashing. This is to allow free-form search of gRNAs against an index. Specificity calculations in such cases should be interpreted carefully of course, since cutting efficiency/specificity are technically defined only for 20mers with NGG PAMs.