Trimming and merging reads from .ab1 Sanger sequencing files.
Part of the human diet quantification project, which aims to establish a model for quantifying dietary components from DNA metabarcoding data.
- Isolate segment with the highest quality from the sequence, using Kadane's algorithm.
- Trim low-quality ends of the sequence.
- Align the forward/reverse PCR products using the Smith-Waterman algorithm for local alignment.
- Merge the reads if the overlap is large enough.
- BLAST the merged sequence.