This plugin provide wrappers around Gctf program.
You will need to use 2.0 version of Scipion to be able to run these protocols. To install the plugin, you have two options:
a) Stable version
scipion installp -p scipion-em-gctf
b) Developer's version
- download repository
git clone https://github.com/scipion-em/scipion-em-gctf.git
scipion installp -p path_to_scipion-em-gctf --devel
Gctf binaries will be installed automatically with the plugin, but you can also link an existing installation.
Default installation path assumed is
software/em/gctf-1.06, if you want to change it, set GCTF_HOME in
scipion.conf file to the folder where the Gctf is installed. Depending on your CUDA version and GPU card compute capability you might want to change the default binary from
Gctf-v1.06_sm_20_cu8.0_x86_64 to a different one by explicitly setting GCTF variable. If you need to use CUDA different from the one used during Scipion installation (defined by CUDA_LIB), you can add GCTF_CUDA_LIB variable to the config file. Various binaries can be downloaded from the official Gctf website.
To check the installation, simply run one of the following Scipion tests:
scipion test gctf.tests.test_protocols_gctf.TestGctfRefine scipion test gctf.tests.test_protocols_gctf.TestGctf
1.06 and 1.18.
1.18 is a special version designed for VPP data, it does not support local/movie CTF refinement or validation options.
- ctf estimation
- ctf refinement
- Zhang K. (2016). Gctf: Real-time CTF determination and correction. JSB, 193: 1-12.