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Documentation says "genome" can be a string but code does not support it #1437
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Session info: > sessionInfo()
R version 4.2.2 (2022-10-31)
Platform: aarch64-apple-darwin20 (64-bit)
Running under: macOS Monterey 12.5.1
Matrix products: default
LAPACK: /Library/Frameworks/R.framework/Versions/4.2-arm64/Resources/lib/libRlapack.dylib
locale:
[1] en_US.UTF-8/en_US.UTF-8/en_US.UTF-8/C/en_US.UTF-8/en_US.UTF-8
attached base packages:
[1] stats4 stats graphics grDevices utils datasets methods
[8] base
other attached packages:
[1] BSgenome.Hsapiens.UCSC.hg38_1.4.5 BSgenome.Hsapiens.UCSC.hg19_1.4.3
[3] JASPAR2020_0.99.10 future_1.32.0
[5] patchwork_1.1.2 ggplot2_3.4.2
[7] EnsDb.Hsapiens.v75_2.99.0 ensembldb_2.22.0
[9] AnnotationFilter_1.22.0 GenomicFeatures_1.50.4
[11] AnnotationDbi_1.60.2 Biobase_2.58.0
[13] SeuratObject_4.1.3 Seurat_4.3.0.1
[15] TFBSTools_1.36.0 Signac_1.10.0
[17] BSgenome_1.66.3 rtracklayer_1.58.0
[19] Biostrings_2.66.0 XVector_0.38.0
[21] GenomicRanges_1.50.2 GenomeInfoDb_1.34.9
[23] IRanges_2.32.0 S4Vectors_0.36.2
[25] BiocGenerics_0.44.0
loaded via a namespace (and not attached):
[1] utf8_1.2.3 spatstat.explore_3.2-1
[3] reticulate_1.30 R.utils_2.12.2
[5] tidyselect_1.2.0 poweRlaw_0.70.6
[7] RSQLite_2.3.1 htmlwidgets_1.6.2
[9] grid_4.2.2 BiocParallel_1.32.6
[11] Rtsne_0.16 munsell_0.5.0
[13] codetools_0.2-19 ica_1.0-3
[15] miniUI_0.1.1.1 withr_2.5.0
[17] spatstat.random_3.1-5 colorspace_2.1-0
[19] progressr_0.13.0 filelock_1.0.2
[21] knitr_1.43 rstudioapi_0.14
[23] ROCR_1.0-11 tensor_1.5
[25] listenv_0.9.0 labeling_0.4.2
[27] MatrixGenerics_1.10.0 GenomeInfoDbData_1.2.9
[29] polyclip_1.10-4 farver_2.1.1
[31] bit64_4.0.5 parallelly_1.36.0
[33] vctrs_0.6.3 generics_0.1.3
[35] xfun_0.39 biovizBase_1.46.0
[37] BiocFileCache_2.6.1 R6_2.5.1
[39] ggbeeswarm_0.7.2 bitops_1.0-7
[41] spatstat.utils_3.0-3 cachem_1.0.8
[43] DelayedArray_0.24.0 promises_1.2.0.1
[45] BiocIO_1.8.0 scales_1.2.1
[47] nnet_7.3-19 beeswarm_0.4.0
[49] gtable_0.3.3 globals_0.16.2
[51] goftest_1.2-3 seqLogo_1.64.0
[53] rlang_1.1.1 RcppRoll_0.3.0
[55] splines_4.2.2 lazyeval_0.2.2
[57] dichromat_2.0-0.1 checkmate_2.2.0
[59] spatstat.geom_3.2-1 yaml_2.3.7
[61] reshape2_1.4.4 abind_1.4-5
[63] backports_1.4.1 httpuv_1.6.11
[65] Hmisc_5.1-0 tools_4.2.2
[67] ellipsis_0.3.2 RColorBrewer_1.1-3
[69] ggridges_0.5.4 Rcpp_1.0.10
[71] plyr_1.8.8 base64enc_0.1-3
[73] progress_1.2.2 zlibbioc_1.44.0
[75] purrr_1.0.1 RCurl_1.98-1.12
[77] prettyunits_1.1.1 rpart_4.1.19
[79] deldir_1.0-9 pbapply_1.7-0
[81] cowplot_1.1.1 zoo_1.8-12
[83] SummarizedExperiment_1.28.0 ggrepel_0.9.3
[85] cluster_2.1.4 motifmatchr_1.20.0
[87] magrittr_2.0.3 data.table_1.14.8
[89] scattermore_1.2 lmtest_0.9-40
[91] RANN_2.6.1 ProtGenerics_1.30.0
[93] fitdistrplus_1.1-11 matrixStats_1.0.0
[95] evaluate_0.21 hms_1.1.3
[97] mime_0.12 xtable_1.8-4
[99] XML_3.99-0.14 gridExtra_2.3
[101] compiler_4.2.2 biomaRt_2.54.1
[103] tibble_3.2.1 KernSmooth_2.23-21
[105] crayon_1.5.2 R.oo_1.25.0
[107] htmltools_0.5.5 later_1.3.1
[109] tzdb_0.4.0 Formula_1.2-5
[111] tidyr_1.3.0 DBI_1.1.3
[113] dbplyr_2.3.2 rappdirs_0.3.3
[115] MASS_7.3-60 Matrix_1.5-4.1
[117] readr_2.1.4 cli_3.6.1
[119] R.methodsS3_1.8.2 parallel_4.2.2
[121] igraph_1.5.0 pkgconfig_2.0.3
[123] GenomicAlignments_1.34.1 TFMPvalue_0.0.9
[125] foreign_0.8-84 sp_2.0-0
[127] plotly_4.10.2 spatstat.sparse_3.0-2
[129] xml2_1.3.4 annotate_1.76.0
[131] vipor_0.4.5 DirichletMultinomial_1.40.0
[133] VariantAnnotation_1.44.1 stringr_1.5.0
[135] digest_0.6.31 sctransform_0.3.5
[137] RcppAnnoy_0.0.20 pracma_2.4.2
[139] CNEr_1.34.0 spatstat.data_3.0-1
[141] rmarkdown_2.22 leiden_0.4.3
[143] fastmatch_1.1-3 htmlTable_2.4.1
[145] uwot_0.1.15 curl_5.0.1
[147] restfulr_0.0.15 shiny_1.7.4
[149] Rsamtools_2.14.0 gtools_3.9.4
[151] rjson_0.2.21 lifecycle_1.0.3
[153] nlme_3.1-162 jsonlite_1.8.5
[155] viridisLite_0.4.2 fansi_1.0.4
[157] pillar_1.9.0 lattice_0.21-8
[159] ggrastr_1.0.2 KEGGREST_1.38.0
[161] fastmap_1.1.1 httr_1.4.6
[163] survival_3.5-5 GO.db_3.16.0
[165] glue_1.6.2 png_0.1-8
[167] bit_4.0.5 stringi_1.7.12
[169] blob_1.2.4 caTools_1.18.2
[171] memoise_2.0.1 dplyr_1.1.2
[173] irlba_2.3.5.1 future.apply_1.11.0 |
timoast
added a commit
that referenced
this issue
Jun 28, 2023
Thanks, this should be fixed now on the develop branch |
Thank you for the really quick response on this! |
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The documentation for
AddMotifs
suggests thegenome
parameter can be "ABSgenome
,DNAStringSet
,FaFile
, or string stating the genome build recognized bygetBSgenome
." (emphasis added by me).However, if we use
'hg19'
or'hg38'
as the value, we run into an error stating:I dug through the code and this probably happens here: https://github.com/stuart-lab/signac/blob/master/R/motifs.R#L45
The string is recognized and processed by
CreateMotifMatrix
:https://github.com/stuart-lab/signac/blob/master/R/preprocessing.R#L142
but the new
genome
object (BSgenome) is not available to the parent function. The assignment needs to happen using a global assignment operator (<<-
) or thegenome
variable needs to be returned to the calling function for the change to reflect.Code to reproduce this from your vignette:
However, this works:
I have not tested the code above but@esouto6 has been running your vignette code on our human data and ran into the error mentioned above, which I think you should be able to reproduce on your vignette data.As this issue was originally reported by my colleague @esouto6, he will provide his
sessionInfo()
in a comment below.EDIT: I've run the vignettes on mouse brain and adding motifs and verified this error.
The text was updated successfully, but these errors were encountered: