Run with "-single x.fastq --run_as_paired"

[YouxinZhao]

When i run trinity with one fastq file which has paired sequences, it showed "Error, found read_type 2 but expecting read_type 1"

/public/home/zengqifan/zhaobaojun/app/trinityrnaseq-v2.14.0/Trinity --seqType fq --max_memory 200G --single x.fastq --run_as_paired

A test file of x.fastq
@ERR12259795X1/1
CGAGGTTTGTAACAAACGCTTTGCGAATGTCTTCAAAGAGGGCCTCAACCTCATGTTTATTGTCCTCATTAAAATTATCCTCCAAGTAGAGATCTCCTAGCGCGTAACCAAGGATAGAGTTTGACGTGGCC
+
JJJFJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJFJJJJJJJJJJFJJJJJJJJJJJJJJJJJJJFJF-7AAJFAF<-FJFJF7JF7AJ-77FJ7AF<J<7-AJFJJ
@ERR12259795X2/1
GCTGAACATCGATTCCACGAGCCAACAAGTCAGTGGTGATGAGTACTCGACTGGATCCAGTACGGAATTCACGCATAATCACGTCTCTCTCCTTCTGGTCCATATCCCCATTCATTGCGGAGACAGTAAAA
+
FJJJJJJJJJJJJJJJJJJJJJJJJJJJJFFJJJJJJJJJJJJJJJJJJJJJJJJFJJJJJJJJJJJJJJJJFJJJJJJJJJJJJJJJFFJJJ-FAAJAJ--AJJF<<FAJ-FAA-<FJA-FJ---7FFJJ
@ERR12259795X3/1
GTCAATCCAAACAATGACTTTAGGATCGTTTATTTGAAGAAAAACACCACTTCTAACCAGCGATCGCTCTTCACGCCTTTTACATGGCGCATTCAGTGGATCACGAGTGACGTCACAGCAAAGAGAAGTAA
+
JJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJFJJ77AFAF7-<-<FJJJ<--777-7FFJAJJJJJ--7AF7JJ
@ERR12259795X4/1
GAACGGCCATGGAATGGTCAAAACGGCCCGATTGTTAATTTTTCAATCAGTCAAAACGGCCCAAATAAAGGTTTACCCTGTTTTAACACTTTAAACCTAAAATGTTGCCGGTTGGACTTATTTCGGGCCGG
+
FJJJFAJJJJ<AJFFFJJFJ7AFFJJFFJJFJFFJJJJJFJFFJJFJJJJ-FJF-AFFFJFJAJFJJFJJFA<7AFJJJJ<AFFJJJ<AJJ-F-FJA--7-A-7FFJ7FF-7FJFJF7-7<FJF7-7AJJ-
@ERR12259795X1/2
TGATTGTTTCAACACCAGCATACTTCTCTAAACTTGGACAGATAAGTACTGGCACAAAGGATCTTACAGTAGCCAATTATATGGTCTGGCAGAGTGTTACTAATCGGTGCGGAGACTTGACGGACAAGGTG
+
FJF<AFFJJJJJAJJJJJJJJFAJFJJJJJJJJ-FA-AF-FA-<<-7-<--AAAFJ------7---7<-7A<<<AF-A--AF--77A--7AJJJ<--<7F7A--77-7AAJ7--77-AF---<--7--7--
@ERR12259795X2/2
TGGACACCCTGAGTGATCTGTACGAGACACTGACTATAACTCAAGCTGTGATCTTCTGTATCAGGTGTAGGAAGGTAGATTGTCTCACAGAGAAAGTGTTTCACAGAGATTTTACTGTCTCCTCCATGTAT
+
JJJJFJJJJJJJFJJJJJJJJJJ<JJ7FFJJJJJJJJJJJJJFAF-<--AAJJF-<FFFJ-FF---A-<777FF-AJA7AAA-7-<FJJ-FFAFJF-7AF-7-A77F--7-7-AF--FFFF--7A--7-77
@ERR12259795X3/2
ACTTCCCTTTGCTGTGACGTCACTCGTGATCCACTGAATGCGCCAGGTAAAAGGCGTGAAGAGCGATCGCTGGTTAGAAGTGGTGTTTTTCTTCACATAAACGAGCCTAAAGTCATTGTTTGGAGTAACAG
+
JJJJJJJJJJJJ<FJJJJJJJJJ7JJAAJJJJJJJJJJFJJFJAF-AFJFFJJJJJ7A77-A-AF-JA77-77AJAJFF<7FFFJFJ-A-7AAAF<JAJJJJAJ-AJ7A-FJ<FJF<AJFF-F<-7--7--
@ERR12259795X4/2
CCGCGTTAATTTCCAAACATCCTAGAACTTCTAGAAGAAGGTATCTAGCGGCACCGGATTGCATACGAAACGGCCCGATGTCGGGATGTCCTACGCGCAAAACGGCCCGAAACAAGGCAAAACGACAATAT
+
JJJJ<<AJFA<JJJJJJJJJJJJJJJAJJJJJJJFJJFJJF77-<-<7J--AJ<J7-F-7-<A-AAJ-AAJ-7A<JJF-7-<<-7FAJ7JF<AJJ<-AJFFFAF-7FFA-AF-7FJ-FFJF-7--7-A7<7