Quality threshold issue with the Sanger Analysis Workflow #28
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The quality plot of that file is supposed to look like this.
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ping @dkandrov Is it ok to open new issues here ? |
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I have also tried to convert manually ab1 file to fastaq with Ugene and the fastq file quality values are all set to |
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Hello, @hadim! About your question of creating issue here, it is not a problem. But our issue tracker is more prefered. |
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it makes sense now, I thought, quality values was computed on the fly from the chromatogram plot... Would it make sense for Ugene to integrate PHRED as an external tool so people can compute quality values when they are missing from chromatograms ? |
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I realized that my sequencing service also provide |
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Here is an example of a quality file provided : |
Thank you. |
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About Another solution is to add supporting |
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About "Import PHRED Qualities" Workflow Element: Using of this element for your data can't help you, because for this element you need quality in PHRED format. Something like this: |
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I am using the university sequencing service platform so I don't know what type of sequencer they're using. About the But since I can download Thank you. |
Using
Tools > Sanger Data Analysis > Read quality control and alignement, in theFASTQ Quality Trimmerbox if I set the quality greater than 0, all the sequences are discarded.I think the FASTQ conversion get rid of the quality or set it to 0.
My input files are chromatograms (
.abi). One example file can be found there : https://drive.google.com/open?id=0Bwt2y0cMI-oyRm1fVHpOcFhMYlUThe text was updated successfully, but these errors were encountered: