Multi-variate Analysis for FLIM

Determination of Coefficient of Variation in complex FLIM datasets under different imaging conditions Main code is run through "streamlinedversion.m." Requries text file input with headers: ImageFile, KIValue, KIConcen, ManualCFDClass, FluorescentDye, Day, ROI, LaserPower, BinNumber, CollectionTime. Not all columns must contain information.

Results

• Large CV is associated with long-lifetime FLIM data

To Run Code (20240320)

• Download python code "crlb.py" written by Christoph Thiele (2021)
• has been edited from the original repository (https://github.com/thielec/CRLB_FL-SMLM/blob/main/crlb.py) to include a call to the tau function
• Download and run matlab code "plot_dye_crlb_cv.m" to generate plot
• specify any folders or python environments - mpmath is required to run the python code
• specify parameters to evaluate crlb (see python code for details)
• load experimental data (mean lifetime and CV of values)