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Unable to convert 10x bam to fastq using bamtofastq and getting folder named as "MissingLibrary" #15

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biobug16 opened this issue Mar 18, 2020 · 2 comments

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@biobug16
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Hi i have a quick question, i have few aligned bam files from single cell RNA Seq data. I want to regenerate fastqs from them. In order to do so i am using 10x's bamtofastq utility and I am also getting fastq files but in the specified path within a folder named “MissingLibrary_1_flowcellName”. I am not sure what does this mean? Why the generated folder is named as missing library.
Does anybody have any idea about this?

The command I used is:
bamtofastq possorted_genome_bam.bam ./sample
Any help will be highly appreciated.

Thanks

@pmarks
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pmarks commented Apr 24, 2020

@biobug16 You'll get "MissingLibrary" in the name of the output folder in some cases when the original run of the pipeline was not able to determine a certain name for the input data. You can ignore this -- the fastq in the output directory should be fine.

@Yijia-Jiang
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@biobug16 You'll get "MissingLibrary" in the name of the output folder in some cases when the original run of the pipeline was not able to determine a certain name for the input data. You can ignore this -- the fastq in the output directory should be fine.

Then how do you set a certain name for the directory of output data? We don't want a missing library output directory with some random letters.

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