Bulk FAST5 used in BulkVis Bioinformatics publication

[Damien-Black]

This is a question,

I'm trying to launch the browser and view the signal data as you do in Figure 2 of BulkVis Paper. Here's what I do:

1. Download the supplementary data you provide HERE
2. Install BulkVis as specified in the installation.
3. Attempt to configure for Collection001 data set.

• I run into an issue when attempting to run set_config.py, I don't know where the bulkfile from the original MinION run is.

Is it possible to get access to the bulkfile so I'm able to properly configure BulkVis?

[alexomics]

Hi Damien,

The bulk FAST5 file used in the paper can be downloaded from http://s3.amazonaws.com/nanopore-human-wgs/bulkfile/PLSP57501_20170308_FNFAF14035_MN16458_sequencing_run_NOTT_Hum_wh1rs2_60428.fast5

This is a 21GB file, so it may take a while to download.

Alex

[Damien-Black]

Thanks @alexomics!

[liu-pinnng]

Hi Alexomics,

I checked the bulk raw output items, however, still could not achieve analyzable bulk FAST5?
Could you tell me how to achieve analyzable bulk FAST5?
Thank you in advance.