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Hi @RajFer, If you know the cell surface area of your experiments and have the Papp at different substrate concentrations, you could in theory calculate Vmax and Km by fitting the data to a michaelis menten equation yourself: CLint (µl/min) ~ Papp (cm/s) * SA (cm²) * 60 (s/min) * 10^(3) (µL/cm^3) Plot V vs [S], fit V = Vmax*[S]/(Km + [S]) The problem is that you then still need your transporter concentration in order to be able to use this value. See e.g. https://github.com/Open-Systems-Pharmacology/Forum/discussions/830 But I am sure the experts here can weigh in much more than I can :) |
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Hi,
I am new to OSP.
Great if somebody clarify me the following:
I have a measured MDCKII-MDR1 transporter Papp value and struggling to how to enter this in PK-Sim.
Tried "Transport Proteins" under "Transport and Excretion" tab but it requires Vamx and Km not the Permeability Coefficient or ER
values you would get in MDCK assays.
Thanks very much in advance.
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