Is the average signal and variance dependent on the length of the flanks to plot?

[ktrns]

Hi,

Another question of mine. I was plotting the same sample around two sets of genes twice, once with 2000 bp flanks and once with 10000 bp flanks. It seems as if the average signal and variance differs even though it is the same data. Can you explain this?

These are my function calls:
getPlotSetArray(tracks="dataset.bw", features=c("genes-class1.bed", "genes-class2.bed"), refgenome="myGenome", xmin=2000, xmax=2000, bin=100)
getPlotSetArray(tracks="dataset.bw", features=c("genes-class1.bed", "genes-class2.bed"), refgenome="myGenome", xmin=10000, xmax=10000, bin=100)

Thanks in advance,
Katrin

[Przemol]

Can you please send me the sample data so I can try to reproduce this behaviour?
You can upload them here, or if they are big - use some file sharing service and post a link.