Required Input Files

Gene Expression Matrix

A tab-delimited text file with transcript abundances.

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Signatures*

A list of gene 'signatures' (annotated gene sets)

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Sample Housekeeping Genes*

A list of housekeeping genes, one gene per line.

Sample Meta-Data (a.k.a "Pre-computed Signatures")*

Meta-data (such as time post-stimulation, genotype, phenotype) for each sample (cell).

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* While it is useful to run FastProject with both, having either a signature file or a pre-computed signature file is sufficient.