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slavseq_rf

Analysis pipeline for human LINE-1 targeted sequencing data obtained with the SLAV-Seq protocol.

Publication

https://doi.org/10.1038/nn.4388

Prerequisites

  • rf
  • pyslavseq
  • snakemake
  • cutadapt
  • bwa-mem
  • tabix
  • python
  • perl

How to use

This pipeline uses rf (https://github.com/apuapaquola/rf) to manage workflow.

  • clone the repository
  • move the pipeline directory to a suitable location
  • edit _h/run scripts or set the BASE_DIR to /path/to/pipeline
  • go to pipeline/genome and rf run -r . to download and index hg19
  • add your fastq files
  • go to pipeline/genome and rf run -r .