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The manuscript doesn’t really mention TPMs, but the help for rosa states that TPMs are required to run the function. I believe it would not be clear to a less experienced user how to calculate a TPM for the anti-sense counts generated on the custom anti-sense annotation.
Sudbery I. Referee Report For: Detection and mitigation of spurious antisense expression with RoSA [version 1; peer review: 1 approved with reservations]. F1000Research 2019, 8:819
(https://doi.org/10.5256/f1000research.20776.r49670)
The text was updated successfully, but these errors were encountered:
The manuscript doesn’t really mention TPMs, but the help for rosa states that TPMs are required to run the function. I believe it would not be clear to a less experienced user how to calculate a TPM for the anti-sense counts generated on the custom anti-sense annotation.
Sudbery I. Referee Report For: Detection and mitigation of spurious antisense expression with RoSA [version 1; peer review: 1 approved with reservations]. F1000Research 2019, 8:819
(https://doi.org/10.5256/f1000research.20776.r49670)
The text was updated successfully, but these errors were encountered: