Error in add(bin) : internal: buf != <newline> in Sample Inference

[Jiayonglai123]

Dear Prof. Ben,

I have been trying to run my Illumina Novaseq NGS V4V5 primer samples with DADA2 in R all scripts run well,
but it shows Error in add(bin) : internal: buf != when running sample inference for
dadaRs <- dada(filtRs, err = errR, multithread = TRUE)

the dataset encounter the same issue in #1869 , where it is unable to plot for quality, where i skip truncating.

Thanks in advance Prof Ben and team

[benjjneb]

To start, can you make this error smaller and more reproducible?
In this case I would say: Can you identify one sample that reproducibly causes this error?
We can then look more closely at that sample/file.

[Jiayonglai123]

A quick update with the error....
I tried separating the samples and run in small batches, it works

it also work for plotQuality where #1869 error when run in small batches,

thus solving all issues.

Thanks for the help and support,
great fan of DADA2 and Prof Ben