ERROR: Cannot find seed sequence in genome of reference species for Dracaena_cambodiana_DN10000_c0_g1_i1.p1!

[majssssa]

I encountered the following error while running fDOG
ERROR: Cannot find seed sequence in genome of reference species for Dracaena_cambodiana_DN10000_c0_g1_i1.p1! This is my code fdog.run --seqFile Dracaena_cambodiana.pep --jobName test --refspec ARATH@3702@3`.Also, nothing appears in the window when I run this command.Finally, the above error occurs.
This is my seqFile

Dracaena_cambodiana_DN10000_c0_g1_i1.p1
MVAVFNKELLSCYLITLKLKQTVEAGLAKSQPNSPTPKPPLRLTQGQPPQPPVPRADSEWVISIREKLDEARQEQAACPWAKLSIYRVPKSLREGDSKAYVPQVVSLGPYHRGKHHLRGMDHHKWRALHKVLRRTGHDVELYLDSIKMLEERARACYEGSLSLTSNEFVETMVLDGIFILELFRGAAGEGFKQLGYSHNDPVFAMRATMHSIQRDMIMLENQIPLFVLDRLLALQICKPEQSGLVATLAVQFFDPLIPTDEPLRKIDRNKLQSTSPSLKATAAVFDP
Dracaena_cambodiana_DN10004_c0_g1_i1.p1
VKEEKLSKDSNSKNGAKSGGELKRASKRPDLKAVSKMIENHQVLYPEKMIGHLPGIDVGDQFLSRAEMCVLGLHNHWLNGIDYMGQSFAKLERYKNYTFPLAICIVLSGMYEDDLDNSEDIVYTGQGGHDLLGSKHQVSDQKMERGNLALKNNCDLGVPVRVVRGHELKSSYSGKVYTYDGLYRVIKTWAEKGVSGFTVYKYKLRRVEGQPVLRTNQVQFTRAT
Dracaena_cambodiana_DN10014_c0_g1_i1.p1
VRLLLLVGPAQVQIQQLLLEKLPEHFDAVSPSRNLNDDVARLIVNQFRWLDFLVDSRGFAEKLMEVLSIAPLLLKKEIIGSLPEIIGDQNSSMVIASLERLLQEDSEVIVPVLDSFSNLNLDDQLQEQAVTIALSCIRTVDAEHMPHLLRFLLLSATSVNVGRIISQIREQLKFVGVSDPCVAKQKMLKGKYLADSTEASILDALRSSLRFKNTTCEAIFKELKSLDHPRDHKVIDVWFLMLIYTNGGSLQKNAEKIL
Dracaena_cambodiana_DN10015_c0_g1_i1.p1
MTPNANTLPPPQTPTPNSLIMAGKSPKPSILTDGLLFFGGAAVALLLFLTLYSFLSPLAPSSPSLPFRHSLPLLANPHSPRHHHNSCDSNLHIDPSSPNLYDDPSVSYTLSYSGSLITDWDRKRTNWLHSHPTFAAKRNRILMVSGSQPGPCRNPIGDHLLLRFYKNKADYCRLHDIDLFYNTALLQPDMFSFWAKIPVVRAAMVAHPEAEWIWWVDSDAAITDMEFELPLDRYEGYNLVLHGWRDLVYKAKSWTSLNAGVFLMRNCQWSLDFIDTWSRMGPQSPEYERWGRIQRAIFKDKLYAASDDQTGIAYLLLEEKRKWADKIYLESAFYFEGYWVEIVNKLERIEAEYLEMDRRVKGLRRRRAEKVAVAYGRMREELLEKQGVRRGMEGWRRPFITHFTGCQPCSGDHNKEYSGENCFGGMQRALNFADNQVLRSYGFRHERLVNSTGVRPLPFDFPAAG*
Dracaena_cambodiana_DN10025_c0_g1_i1.p1
MSPCGGVGHEPAVLQLQKWGYLQFQLEPSEFRLASISPTRDLLLLLSYQCEALLLPLLLGKFQSENICEPNSSEQVITCIPDDVDSAQCSKNDEESVKGAALPILESSSASKSYPVISGVKSLAWGHS
Dracaena_cambodiana_DN10026_c0_g1_i1.p1
VICLGIFFRSWPSPRPSFIRDISQQNSSESYGLVHVCALGGEFLCRLAVFAILERLGSQILYSMFASFCLMAAIFLRRNIVETKGKTLQEIEISLRQPE*
Dracaena_cambodiana_DN10027_c0_g1_i1.p4
MSPTDRGGPTSSAVGSLHLLPSELLHSILLRLPLPDLLRLRSLSRLLLSSISAPEFRRTYQSTSPWLFLFQKRPPRSSLLRGFDPRSARWFTFPSLSALIAAPALPPGDDLYLLA
Dracaena_cambodiana_DN10028_c0_g1_i1.p1
MGFTAAELERLISSNARILIVAPAIPRLEFWQAFIGGDNRKDLVSVLTRNRGLITHDIVSGIAPKMLLLKEHGLSQRDIVGLVKRGHGFITRSSKTIEAVLNSAKELGLDTKSPMFGHTLSSLVSFSSDSFKAKMEIFRGFGWSEEELLAAFKKAPSFLHLSEENIREKMEFLVGRAGCKQSYIALNPLLLTFSLEKRLRPRHYVMEVLKSKGIMGRARFSKIMCLTEKKFVESVILPCKEQVPNIHELYIA
Dracaena_cambodiana_DN10030_c0_g1_i1.p1
LFRNPLSLSLLRKFFPKQISNLMGYLSCRAESSISTCRSISAISPSQSPTSTTNTKKKHLKPYKSLQNQENDDDDDDKSPCRRIEHFTYAELESATNNFSNSSLLGRGS

[trvinh]

Please prefer to this wiki to make sure you have the correct input data for fDOG https://github.com/BIONF/fDOG/wiki/Input-and-Output-Files (and https://github.com/BIONF/fDOG/wiki/Use-standalone-version)

• For fdog.run, it must be a SINGLE sequence in FASTA format
• The reference species (specified with --refspec) must be present in the blast database directory

Also check this page https://github.com/BIONF/fDOG/wiki/How-fDOG-works to know the differences between core taxa and search taxa. I think you are mistaken between these, since you said that your reference species is Dracaena_cambodiana_DN10000_c0_g1_i1.p1 but you have set --refspec ARATH@3702@3.

[trvinh]

@majssssa This issue is solved, right?

[majssssa]

I'm sorry to see your reply just now. This problem has been successfully solved

[trvinh]

Nice! Then I will close it :)