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Revamp of metagenomics rules #927

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Revamp of metagenomics rules #927

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yesimon
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@yesimon yesimon commented Feb 15, 2019

Add support for krakenuniq, kaiju to replace kraken and diamond in default pipelines.

Both rely on customized krakenuniq/kaiju packages for custom functionality, like loading database
from pipe or printing out all taxa hits (kaiju).

Krona fixed to use hit counts as scores in report files directly, instead of counting the
individual read classifications (which takes a long time).

Includes changes from #915 and also some pytest deprecation fixes: s/log.warn(/log.warning(/, runslow command line configuration.

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dpark01 commented Feb 20, 2019

Fixes #777.

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dpark01 commented Feb 20, 2019

Do we know how this fares with single-end read BAM input? #820

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Since this heavily relies on yesimon-specific forks of krakenuniq and kaiju, can you send us links to the code diffs between the forks and the upstream/authoritative? In your github I see forks where your master branches have not diverged from the upstream master branches, so I assume the magic is all in one of the named branches of each repo?

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yesimon commented Mar 1, 2019

The fork of krakenuniq is the sy-multiple branch: https://github.com/yesimon/krakenuniq/tree/sy-multiple.

The fork of kaiju is minor and is in the sy-report-viruses branch: https://github.com/yesimon/kaiju/tree/sy-report-viruses

@yesimon yesimon force-pushed the sy-krakenuniq branch 3 times, most recently from 0a529e1 to 0b0a85f Compare March 3, 2019 00:57
@yesimon yesimon force-pushed the sy-krakenuniq branch 5 times, most recently from 8140a60 to ddff459 Compare March 29, 2019 18:41
Add support for krakenuniq, kaiju to replace kraken and diamond in
default pipelines.

Both rely on customized krakenuniq/kaiju packages for custom
functionality, like loading database
from pipe or printing out all taxa hits (kaiju).

Krona fixed to use hit counts as scores in report files directly,
instead of counting the
individual read classifications (which takes a long time).
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yesimon commented Apr 12, 2019

Incorporated in #944

@yesimon yesimon closed this Apr 12, 2019
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2 participants