Skip to content
New issue

Have a question about this project? Sign up for a free GitHub account to open an issue and contact its maintainers and the community.

By clicking “Sign up for GitHub”, you agree to our terms of service and privacy statement. We’ll occasionally send you account related emails.

Already on GitHub? Sign in to your account

Change SSU search strategy #3

Closed
abremges opened this issue Apr 27, 2018 · 2 comments
Closed

Change SSU search strategy #3

abremges opened this issue Apr 27, 2018 · 2 comments
Assignees
Labels
question Further information is requested
Milestone

Comments

@abremges
Copy link
Member

Currently, we use Barrnap (which is great and simple to use), but always assume domain Bacteria. This results in suboptimal annotations for e.g. Archaea. Either:

  • Check other annotation results for most likely domain and adjust the command line call, or
  • Use CheckM's ssuFinder module, which searches for all three domains and merges annotations.

I'm slightly leaning towards CheckM at the moment, which will also eliminate the dependencies on Barrnap and Bedtools, but this needs proper testing obviously!

@abremges abremges self-assigned this Apr 27, 2018
@abremges
Copy link
Member Author

Or go big and simply run Prokka on the full genome (w/ known domain from e.g. the conservative CheckM lineage)

@abremges abremges added the question Further information is requested label Apr 30, 2018
@abremges abremges added this to the v1.0 milestone Apr 30, 2018
@abremges
Copy link
Member Author

Switched to checkm ssu_finder

Sign up for free to join this conversation on GitHub. Already have an account? Sign in to comment
Labels
question Further information is requested
Projects
None yet
Development

No branches or pull requests

1 participant