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T-Coffee Tutorials | ||
******************* | ||
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Using the T-Coffee Package to Build Multiple Sequence Alignments of Proteins, RNAs, DNA | ||
sequences and 3D-Structures. | ||
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Introduction | ||
--------------- | ||
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T-Coffee is a versatile Multiple Sequence Alignment method suitable for aligning most types | ||
of biological sequences. The series of protocols presented here show how the package can be | ||
used to multiply align proteins, DNA and RNA sequences. | ||
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The protein section presents controlled cases for PSI-Coffee the homology extended mode suitable | ||
for remote homologues, Expresso the structure based multiple aligner and M-Coffee, a meta-version | ||
able to combine several third party aligners into one. | ||
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We then show how the T-RMSD option can be used to produce a functionally informative structure | ||
based clustering. RNA alignment procedures are shown for R-Coffee a mode that produces secondary | ||
structure based MSAs. DNA alignments are illustrated with Pro-Coffee, a multiple aligner specific | ||
of promoter regions. The last sections presents the many reformatting utilities bundled with T-Coffee. | ||
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The package is an open-source freeware available from `www.tcoffee.org <http://www.tcoffee.org>`_. | ||
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Materials | ||
----------- | ||
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The list of files (input and output) required by this protocol is available | ||
from `here <http://www.tcoffee.org/Packages/NatureProtocols/NatureProtocolDataset.tar.gz>`_. | ||
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They can be automatically retrieved using the following command:: | ||
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t_coffee -other_pg nature_protocol.pl | ||
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This will create 4 repertories containing the input sequences necessary for the protocols | ||
we report in this section. For each part, all command lines have been collected into the file README.sh. | ||
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Procedures | ||
------------ | ||
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- Installation | ||
- Protein Multiple Sequence Alignments | ||
- RNA Multiple Sequence Alignments | ||
- Promoter alignments | ||
- Reformat alignments |