Counts file - only relative abundance data

[davitero]

Hello,
My data are only OTUs and their relative abundances because they were obtained in a previous investigation.

So I was wondering, can I work with my relative abundance data instead of read counts? That is, can I use this information as count file?

[wevanjohnson]

Yes, you can, multiple, your abundances by a large number, say 100000 and then round to the nearest integer, and treat them as counts. You can use any of the functions that use relative abundance just fine. But be careful with anything that uses the counts directly, e.g. DESeq2 on these data.

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On Oct 10, 2020, at 12:31 PM, davitero ***@***.***> wrote: Hello, My data are only OTUs and their relative abundances because they were obtained in a previous investigation. So I was wondering, can I work with my relative abundance data instead of read counts? That is, can I use this information as count file? — You are receiving this because you are subscribed to this thread. Reply to this email directly, view it on GitHub <#43>, or unsubscribe <https://github.com/notifications/unsubscribe-auth/ACMBWPFGLPHQBARILQVMWSTSKCD4JANCNFSM4SLFIWQA>.

[davitero]

Dear Evan,
Thank you for your answer. I will do as you say.
Best,
Dave