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GTC files cannot be listed through both command interface and file list when only submitting a .txt file #48
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The problem is that your
Notice that using the option |
Thank you for the quick response!. That seemed to fix that, but now I' getting this: "Header of file ./ProjectDetailReport ILMN GDA 07-11-22 AMS1.csv is incorrect:". The csv is attached. Thank you |
Ah yeah, the manifest file name did seem weird to me. That's because |
I think I got it working using the _A2.csv.gz. I'm using the _A2.bpm. |
Hi,
I am getting the error message "GTC files cannot be listed through both command interface and file list" even though I am only submitting a single .txt file with a list of the gtc file names. I have tried this where the actual gtc files are in the directory where I am running the script, and also where they are in their own directory. I am running on a google cloud instance and using a singularity container. Here is the code, and I have attached the gtc_list file.
`bpm_manifest_file="./GDA_PGx-8v1-0_20042614_A2.bpm"
csv_manifest_file="./ProjectDetailReport ILMN GDA 07-11-22 AMS1.csv"
egt_cluster_file="./GDA FINAL 3 plate validation reclustered 06302022.egt"
path_to_gtc_folder="./gtc_file_list.csv"
ref="./GRCh38_full_analysis_set_plus_decoy_hla.fa" # or ref="$HOME/GRCh37/human_g1k_v37.fasta"
out_prefix="206486390022"
singularity exec gtc2vcf_072922.sif bcftools +gtc2vcf
--no-version -Ou
--bpm $bpm_manifest_file
--csv $csv_manifest_file
--egt $egt_cluster_file
--gtcs ./gtc_list_file.txt
--fasta-ref $ref
--output $out_prefix.vcf
--output-type v
--extra $out_prefix.tsv
--verbose
`
Thank you
Harry
gtc_list_file.txt
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