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gtf2gff error #9

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tiramisutes opened this issue Oct 31, 2018 · 7 comments
Closed

gtf2gff error #9

tiramisutes opened this issue Oct 31, 2018 · 7 comments
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@tiramisutes
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Dear,
I have downloading the latest version of BRAKER2 and runing wiht --gff3 flag. But I get follows stderr in gtf output file convert to gff3 format.

ERROR in file /home/software/BRAKER/scripts/braker.pl at line 9415
Failed to execute: cat /home/results/BRAKER/augustus.hints.gtf | perl -ne 'if(m/\tAUGUSTUS\t/) {print $_;}' | perl /home/software/Augustus/scripts/gtf2gff.pl --gff3 --out=/home/results/BRAKER/augustus.hints.gff3 >> /home/results/BRAKER/gtf2gff3.log 2>> /home/results/BRAKER/errors/gtf2gff3.err

and gtf2gff3.err file show

transcript jg1.t1 has conflicting gene parents: and jg1. Remember: In GTF txids need to be overall unique. at /home/software/Augustus/scripts/gtf2gff.pl line 119, <STDIN> line 590303.

Any help is much appreciated.
Thanks.

@francicco
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Same error...

@KatharinaHoff
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This will be solved when the Augustus issue Gaius-Augustus/Augustus#31 is fixed.

@ayala-usma
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ayala-usma commented Nov 15, 2018

I am having the same error. And when I look at the gtf2gff3.err file in errors folder, the output is:
transcript jg1.t1 has conflicting gene parents: and jg1. Remember: In GTF txids need to be overall unique. at /path/to/Augustus/scripts/gtf2gff.pl line 120, <STDIN> line 137778.

@HSKim3824
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I also have same problem.

perl /BiO/Access/hskim3824/bioapps/Augustus/scripts/gtf2gff.pl <augustus.hints.gtf --out test.gff3

transcript jg1.t1 has conflicting gene parents: and jg1. Remember: In GTF txids need to be overall unique. at /BiO/Access/hskim3824/bioapps/Augustus/scripts/gtf2gff.pl line 120, line 612516.

so... I could not get gff3 output.

@peterthorpe5
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same error. @KatharinaHoff do you now if there is a fix for the gtf2gff.pl script?

@KatharinaHoff
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A solution for running gtf2gff.pl, manually, has been posted at Gaius-Augustus/Augustus#31 . You'll have to update your Augustus from github in order to obtain the fix_joingenes_gtf.pl script. The "fix" has not been incorporated into the braker.pl pipeline, yet.

@peterthorpe5
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peterthorpe5 commented Jan 18, 2019 via email

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