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De novo transcriptome assembly, annotation, and differential expression analysis #1351

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merged 22 commits into from
Feb 13, 2020

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abretaud
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Finally a tutorial for de novo transcriptome assembly, annotation, and differential expression analysis as promised in #59 .

It's different than the existing de novo transcriptomics as here we don't use a reference genome, we just assemble the transcriptome using Trinity

We (with @r1corre) have a first draft of workflow, but a few things are missing:

  • blast & co process before trinotate
  • trinotate visualization
  • better handling of data collections probably
  • find a good dataset
  • ...

Fixes #59

@hexylena
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@abretaud is this still in WIP?

@lecorguille
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It's still in progress.

TODO :

  • Finish DE part
  • Annotation part
  • Text, Text, Question ...
  • Main questions
  • Main objectives
  • Main key_points
  • Satellite
    • data-library.yaml
    • tutorial.bib
  • ...
  • Full reading

@lecorguille lecorguille changed the title [WIP] De novo transcriptome assembly, annotation, and differential expression analysis De novo transcriptome assembly, annotation, and differential expression analysis Feb 13, 2020
@lecorguille
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Can we consider to merge this PR?
I disabled the tutorial to get a nice URL we will be able to share tomorrow.

I promise to daily beat @r1corre to make him finish this tutorial 👿

bgruening
bgruening previously approved these changes Feb 13, 2020
@@ -540,6 +546,10 @@ workflow4metabolomics:
wm75:
name: Wolfgang Maier

xiliu:
name: Xi Liu
github: false
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can you remove this @lecorguille

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AH ... how can I add Xi as author?

@shiltemann shiltemann merged commit cf64349 into galaxyproject:master Feb 13, 2020
@shiltemann
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thanks @abretaud and @lecorguille! and good luck tomorrow!

@lecorguille
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Thanks!

@vasudhajain29
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hi, I am trying to run denovo transcriptome pipeline on usegalaxy.org, but the issue is I cant see Trinity in the assembly method. Can you please guide me through how to use it?

Thanks

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RNASeq: de novo transcriptome assembly with Trinity
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