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Update junctions-annotate.md
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yang-yangfeng committed Dec 1, 2020
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Expand Up @@ -51,22 +51,23 @@ donors and junctions in the transcriptome. The known acceptor, donor and junctio
information is computed from the GTF file and this information is then used to annotate the observed
junctions.

The junctions are annotated using the following nomenclature(and as shown in the figure below.)
The junctions are annotated using the following nomenclature (and as shown in the figure below.)

1. DA - This exon-exon junction is present in the transcriptome provided by the user(GTF)
The ends of this junction are hence known donor and known acceptor sites according to the GTF file.
1. DA - The ends of this junction are known donor and known acceptor sites according to "annotations.gtf".
This junction is known to the transcriptome.

2. NDA - This exon-exon junction is not present(novel) in the transcriptome provided by the user(GTF)
The ends of this junction are known donor and known acceptor sites according to the GTF file.

3. D - This exon-exon junction is not present(novel) in the transcriptome provided by the user(GTF)
The donor of this junction is a known donor but the acceptor is novel.
2. NDA - The ends of this junction are known donor and known acceptor sites, according to "annotations.gtf".
This junction is not known to the transcriptome (novel).

4. A - This exon-exon junction is not present(novel) in the transcriptome provided by the user(GTF)
The acceptor of this junction is a known acceptor but the donor is novel.
3. D - The ends of this junction are a known donor site and a novel acceptor site, according to "annotations.gtf".
This junction is not known to the transcriptome (novel).

5. N - This exon-exon junction is not present(novel) in the transcriptome provided by the user(GTF)
The ends of this junction are hence not known donor/acceptor sites according to the GTF file.
4. A - The ends of this junction are a novel donor site and a known acceptor site, according to "annotations.gtf".
This junction is not known to the transcriptome (novel).

5. N - The ends of this junction are a novel donor site and a novel acceptor site, according to "annotations.gtf".
This junction is not known to the transcriptome (novel).


![Anchor-annotation example][anchor_annotation]
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